Construct: ORF ccsbBroad304_00480

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015781.1_s304c1, BRDN0000399090
Derived from:
ccsbBroadEn_00480
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EEF1A1 (1915)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00480
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1915 EEF1A1 eukaryotic translation elon... NM_001402.6 100% 100%
2 human 1917 EEF1A2 eukaryotic translation elon... NM_001958.5 77.8% 92.2% (many diffs)
3 human 106635683 SNORD141A small nucleolar RNA, C/D bo... NR_132980.1 7.5% 0_1ins944;105_106ins337
4 human 106635684 SNORD141B small nucleolar RNA, C/D bo... NR_132981.1 7.5% 0_1ins944;105_106ins337
5 mouse 13627 Eef1a1 eukaryotic translation elon... NM_010106.2 91.8% 99.7% (many diffs)
6 mouse 13628 Eef1a2 eukaryotic translation elon... NM_007906.2 79.4% 92.2% (many diffs)
7 mouse 625054 Gm6548 predicted gene 6548; eukary... NR_003363.1 59.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1452
ORF length:
1386
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg aaaggaaaag actcatatca acattgtcgt cattggacac gtagattcgg
121gcaagtccac cactactggc catctgatct ataaatgcgg tggcatcgac aaaagaacca
181ttgaaaaatt tgagaaggag gctgctgaga tgggaaaggg ctccttcaag tatgcctggg
241tcttggataa actgaaagct gagcgtgaac gtggtatcac cattgatatc tccttgtgga
301aatttgagac cagcaagtac tatgtgacta tcattgatgc cccaggacac agagacttta
361tcaaaaacat gattacaggg acatctcagg ctgactgtgc tgtcctgatt gttgctgctg
421gtgttggtga atttgaagct ggtatctcca agaatgggca gacccgagag catgcccttc
481tggcttacac actgggtgtg aaacaactaa ttgtcggtgt taacaaaatg gattccactg
541agccacccta cagccagaag agatatgagg aaattgttaa ggaagtcagc acttacatta
601agaaaattgg ctacaacccc gacacagtag catttgtgcc aatttctggt tggaatggtg
661acaacatgct ggagccaagt gctaacatgc cttggttcaa gggatggaaa gtcacccgta
721aggatggcaa tgccagtgga accacgctgc ttgaggctct ggactgcatc ctaccaccaa
781ctcgtccaac tgacaagccc ttgcgcctgc ctctccagga tgtctacaaa attggtggta
841ttggtactgt tcctgttggc cgagtggaga ctggtgttct caaacccggt atggtggtca
901cctttgctcc agtcaacgtt acaacggaag taaaatctgt cgaaatgcac catgaagctt
961tgagtgaagc tcttcctggg gacaatgtgg gcttcaatgt caagaatgtg tctgtcaagg
1021atgttcgtcg tggcaacgtt gctggtgaca gcaaaaatga cccaccaatg gaagcagctg
1081gcttcactgc tcaggtgatt atcctgaacc atccaggcca aataagcgcc ggctatgccc
1141ctgtattgga ttgccacacg gctcacattg catgcaagtt tgctgagctg aaggaaaaga
1201ttgatcgccg ttctggtaaa aagctggaag atggccctaa attcttgaag tctggtgatg
1261ctgccattgt tgatatggtt cctggcaagc ccatgtgtgt tgagagcttc tcagactatc
1321cacctttggg tcgctttgct gttcgtgata tgagacagac agttgcggtg ggtgtcatca
1381aagcagtgga caagaaggct gctggagctg gcaaggtcac caagtctgcc cagaaagctc
1441agaaggctaa atgcgcaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1501tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1561caatcaacct ctggatta

Download FASTA (ORF) (Full)

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