Construct: ORF ccsbBroad304_00522
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002022.1_s304c1, BRDN0000410756
- Derived from:
- ccsbBroadEn_00522
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ETV6 (2120)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00522
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2120 | ETV6 | ETS variant transcription f... | NM_001987.5 | 93.8% | 56.1% | (many diffs) |
| 2 | human | 2120 | ETV6 | ETS variant transcription f... | XM_011520607.2 | 93.6% | 55.9% | (many diffs) |
| 3 | human | 2120 | ETV6 | ETS variant transcription f... | XM_011520608.2 | 91.7% | 53.7% | (many diffs) |
| 4 | human | 2120 | ETV6 | ETS variant transcription f... | XM_017018990.1 | 83.9% | 46.4% | (many diffs) |
| 5 | human | 2120 | ETV6 | ETS variant transcription f... | XM_011520609.2 | 74.4% | 37.1% | (many diffs) |
| 6 | human | 2120 | ETV6 | ETS variant transcription f... | XM_011520611.2 | 74.4% | 37.1% | (many diffs) |
| 7 | human | 2120 | ETV6 | ETS variant transcription f... | XM_017018991.1 | 74.4% | 37.1% | (many diffs) |
| 8 | human | 2120 | ETV6 | ETS variant transcription f... | XM_011520612.2 | 48.2% | 11.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1005
- ORF length:
- 939
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgagactcct gctcagtgta gcattaagca ggaacgaatt tcatatacac 121 ctccagagag cccagtgccg agttacgctt cctcgacgcc acttcatgtt ccagtgcctc 181 gagcgctcag gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa 241 tttactggag cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt 301 taaggccaat tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca 361 aagaggactt tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc 421 atattctgaa gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact 481 ctatacacac acagccggag gtcatactgc atcagaacca tgaagaagat aactgtgtcc 541 agaggacccc caggccatcc gtggataatg tgcaccataa ccctcccacc attgaactgt 601 tgcaccgctc caggtcacct atcacgacaa atcaccggcc ttctcctgac cccgagcagc 661 ggcccctccg gtcccccctg gacaacatga tccgccgcct ctccccggct gagagACATC 721 AGAGACCCGG CCGCACCGAG GAGAACAACC ACCAGGAGTC CTCTCCTCTG TCAGTGTCTC 781 CCATAGAAAA TAATCAATGC CCAGCGTCTT CCGAGTCCCC CCCGAAGCCA TCCAGCCCCC 841 CGGCAAAAGA GCACACGCGT GATCCAGCTG ATGACCAGCC CCATCATGCA CCCTCTGATC 901 ATGAACCCCA GGCACTCCGT GGATTTAAAA ACAATCCAGG CTCTCCGAGG ATGGGCTGCA 961 TAGGGAAGGG AAGCCCATCA ACGTCTATCA TGGGGAAGAC TTGCTGACAT GAACCACATC 1021 ATGGTCTCTG TCTCCCCGCC TGAAGAGCAC GCCATGCCCA TTGGGAGAAT AGCAGACTGT 1081 AGACTGCTTT GGATTACGTC TATCAGATGA TTTGAGACAG CAGGTACGAA ATCTTCATCC 1141 GATGGGAGGA CAATGAATCC AAAATAATCT GAATAGTGGA TCCCAAAAGA CTAAATCGAT 1201 AGAAGGGAAA CCCTAAGAAC AGAACAAACA TGACAGGGGG AAAATGTCCA GGAGCCCTGA 1261 GAACTTAACG AATAAAACAT TATTGGAAGG AACCAGGACA AAGACTAGAG TCAGGTTGAT 1321 GAAAACCCCA TATGAATTCA TGAGTGGATG AACAGACCGT CTGGAGCCCC TAGACTCCCC 1381 GGAGCTGGAT GAACAAATAA ACCAAGAAGA TGAATACTGC CCAACTTTTT TTTAAAAAGA 1441 ACCATTTTTG AATACAAATT TAAGAAAGAT CCAATTTTTT TCTTATGTAT TCTTTCCTTT 1501 TCATGGAATA GAAAACGTTA ATAAAATATA TTAAATAATT GCTATTACA