Transcript: Human XM_017018991.1

PREDICTED: Homo sapiens ETS variant transcription factor 6 (ETV6), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ETV6 (2120)
Length:
7279
CDS:
1845..2939

Additional Resources:

NCBI RefSeq record:
XM_017018991.1
NBCI Gene record:
ETV6 (2120)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017018991.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003852 AGGAGCTGGATGAACAAATAT pLKO.1 2899 CDS 100% 15.000 10.500 N ETV6 n/a
2 TRCN0000231234 GCATTAAGCAGGAACGAATTT pLKO_005 210 5UTR 100% 13.200 9.240 N Etv6 n/a
3 TRCN0000003853 GCGCCACTACTACAAACTAAA pLKO.1 2774 CDS 100% 13.200 9.240 N ETV6 n/a
4 TRCN0000338363 GCGCCACTACTACAAACTAAA pLKO_005 2774 CDS 100% 13.200 9.240 N ETV6 n/a
5 TRCN0000003856 CCATAAGAACAGAACAAACAT pLKO.1 2726 CDS 100% 5.625 3.938 N ETV6 n/a
6 TRCN0000338303 CCATAAGAACAGAACAAACAT pLKO_005 2726 CDS 100% 5.625 3.938 N ETV6 n/a
7 TRCN0000003854 GTTGTTAGTATCATGGTGTTT pLKO.1 3233 3UTR 100% 4.950 3.465 N ETV6 n/a
8 TRCN0000338364 GTTGTTAGTATCATGGTGTTT pLKO_005 3233 3UTR 100% 4.950 3.465 N ETV6 n/a
9 TRCN0000003855 GCTGCTGACCAAAGAGGACTT pLKO.1 1865 CDS 100% 4.050 2.835 N ETV6 n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1109 5UTR 100% 4.950 2.475 Y KAAG1 n/a
11 TRCN0000162548 CACACACACACACACAAATAT pLKO.1 1113 5UTR 100% 15.000 7.500 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017018991.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00522 pDONR223 100% 80.5% 80.5% None 0_1ins264 n/a
2 TRCN0000475139 CCTCCGCACGACCGATTCAGTGTC pLX_317 21.2% 80.5% 80.5% V5 0_1ins264 n/a
3 ccsbBroad304_00522 pLX_304 51.9% 74.4% 37.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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