Construct: ORF ccsbBroad304_00570
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013057.1_s304c1, BRDN0000410061
- Derived from:
- ccsbBroadEn_00570
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VEGFD (2277)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00570
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2277 | VEGFD | vascular endothelial growth... | NM_004469.5 | 100% | 100% | |
| 2 | human | 100532742 | PIR-FIGF | PIR-FIGF readthrough | NR_037859.2 | 39.6% | (many diffs) | |
| 3 | mouse | 14205 | Vegfd | vascular endothelial growth... | NM_010216.2 | 84.7% | 83.8% | (many diffs) |
| 4 | mouse | 14205 | Vegfd | vascular endothelial growth... | NM_001308489.1 | 75.5% | 75.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta cagagagtgg gtagtggtga atgttttcat gatgttgtac gtccagctgg 121 tgcagggctc cagtaatgaa catggaccag tgaagcgatc atctcagtcc acattggaac 181 gatctgaaca gcagatcagg gctgcttcta gtttggagga actacttcga attactcact 241 ctgaggactg gaagctgtgg agatgcaggc tgaggctcaa aagttttacc agtatggact 301 ctcgctcagc atcccatcgg tccactaggt ttgcggcaac tttctatgac attgaaacac 361 taaaagttat agatgaagaa tggcaaagaa ctcagtgcag ccctagagaa acgtgcgtgg 421 aggtggccag tgagctgggg aagagtacca acacattctt caagccccct tgtgtgaacg 481 tgttccgatg tggtggctgt tgcaatgaag agagccttat ctgtatgaac accagcacct 541 cgtacatttc caaacagctc tttgagatat cagtgccttt gacatcagta cctgaattag 601 tgcctgttaa agttgccaat catacaggtt gtaagtgctt gccaacagcc ccccgccatc 661 catactcaat tatcagaaga tccatccaga tccctgaaga agatcgctgt tcccattcca 721 agaaactctg tcctattgac atgctatggg atagcaacaa atgtaaatgt gttttgcagg 781 aggaaaatcc acttgctgga acagaagacc actctcatct ccaggaacca gctctctgtg 841 ggccacacat gatgtttgac gaagatcgtt gcgagtgtgt ctgtaaaaca ccatgtccca 901 aagatctaat ccagcacccc aaaaactgca gttgctttga gtgcaaagaa agtctggaga 961 cctgctgcca gaagcacaag ctatttcacc cagacacctg cagctgtgag gacagatgcc 1021 cctttcatac cagaccatgt gcaagtggca aaacagcatg tgcaaagcat tgccgctttc 1081 caaaggagaa aagggctgcc caggggcccc acagccgaaa gaatccttgc ccaactttct 1141 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 1201 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta