Construct: ORF ccsbBroad304_00637

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015090.1_s304c1, BRDN0000403512
Derived from:
ccsbBroadEn_00637
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBLIF (2694)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00637
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2694 CBLIF cobalamin binding intrinsic... NM_005142.3 100% 100%
2 human 2694 CBLIF cobalamin binding intrinsic... XM_011544939.3 96.6% 96.6% 828_829ins42
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1317
ORF length:
1251
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ctggtttgcc ctctacctcc tgagccttct ctgggctaca gctgggacta
121gtacccagac ccagagttca tgctccgttc cctcagcaca ggagcccttg gtcaatggaa
181tacaagtact catggagaac tcggtgactt catcagccta cccaaacccc agcatcctga
241ttgccatgaa tctggccgga gcctacaact tgaaggccca gaagctcctg acttaccagc
301tcatgtccag cgacaacaac gatctaacca ttgggcagct cggcctcacc atcatggccc
361tcacctcctc ctgccgagac cctggggata aagtatccat tctacaaaga caaatggaga
421actgggcacc ttccagcccc aacgctgaag catcagcctt ctatgggccc agtctagcga
481tcttggcact gtgccagaag aactctgagg cgaccttgcc gatagccgtc cgctttgcca
541agaccctgct ggccaactcc tctcccttca atgtagacac aggagcaatg gcaaccttgg
601ctctgacctg tatgtacaac aagatccctg taggttcaga ggaaggttac agatccctgt
661ttggtcaggt actaaaggat attgtggaga aaatcagcat gaagatcaaa gataatggca
721tcattggaga catctacagt actggcctcg ccatgcaggc tctctctgta acacctgagc
781catctaaaaa ggaatggaac tgcaagaaga ctacggatat gatactcaat gagattaagc
841aggggaaatt ccacaacccc atgtccattg ctcaaatcct cccttccctg aaaggcaaga
901catacctaga tgtgccccag gtcacttgta gtcctgatca tgaggtacaa ccaactctac
961ccagcaaccc tggccctggc cccacctctg catctaacat cactgtcata tacaccataa
1021ataaccagct gaggggggtt gagctgctct tcaacgagac catcaatgtt agtgtgaaaa
1081gtgggtcagt gttacttgtt gtcctagagg aagcacagcg caaaaatcct atgttcaaat
1141ttgaaaccac aatgacatct tggggccttg tcgtctcttc tatcaacaat atcgcggaaa
1201atgttaatca caagacatac tggcagtttc ttagtggtgt aacacctttg aatgaagggg
1261ttgctgacta catacccttc aaccacgagc acatcacagc caatttcaca cagtactacc
1321caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1381ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1441tta

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