Construct: ORF ccsbBroad304_00722

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005122.1_s304c1, BRDN0000402922
Derived from:
ccsbBroadEn_00722
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HSD17B10 (3028)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00722
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3028 HSD17B10 hydroxysteroid 17-beta dehy... NM_001037811.2 100% 100%
2 human 3028 HSD17B10 hydroxysteroid 17-beta dehy... NM_004493.3 96.5% 96.5% 567_593del
3 mouse 15108 Hsd17b10 hydroxysteroid (17-beta) de... NM_016763.2 82.8% 84.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
822
ORF length:
756
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agcagcgtgt cggagcgtga agggcctggt ggcggtaata accggaggag
121cctcgggcct gggcctggcc acggcggagc gacttgtggg gcagggagcc tctgctgtgc
181ttctggacct gcccaactcg ggtggggagg cccaagccaa gaagttagga aacaactgcg
241ttttcgcccc agccgacgtg acctctgaga aggatgtgca aacagctctg gctctagcaa
301aaggaaagtt tggccgtgtg gatgtagctg tcaactgtgc aggcatcgcg gtggctagca
361agacgtacaa cttaaagaag ggccagaccc ataccttgga agacttccag cgagttcttg
421atgtgaatct catgggcacc ttcaatgtga tccgcctggt ggctggtgag atgggccaga
481atgaaccaga ccagggaggc caacgtgggg tcatcatcaa cactgccagt gtggctgcct
541tcgagggtca ggttggacaa gctgcatact ctgcttccaa ggggggaata gtgggcatga
601cactgcccat tgctcgggat ctggctccca taggtctgtt tggcacccca ctgctgacca
661gcctcccaga gaaagtgtgc aacttcttgg ccagccaagt gcccttccct agccgactgg
721gtgaccctgc tgagtatgct cacctcgtac aggccatcat cgagaaccca ttcctcaatg
781gagaggtcat ccggctggat ggggccattc gtatgcagcc ttgcccaact ttcttgtaca
841aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
901ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)