Construct: ORF ccsbBroad304_00748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012297.1_s304c1, BRDN0000408984
- Derived from:
- ccsbBroadEn_00748
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MR1 (3140)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00748
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3140 | MR1 | major histocompatibility co... | NM_001194999.1 | 100% | 100% | |
| 2 | human | 3140 | MR1 | major histocompatibility co... | NM_001531.2 | 86.8% | 86.8% | 327_461del |
| 3 | human | 3140 | MR1 | major histocompatibility co... | XM_011509463.2 | 80.4% | 73.4% | (many diffs) |
| 4 | human | 3140 | MR1 | major histocompatibility co... | XM_024446611.1 | 78.2% | 78.2% | 327_461del;601_602ins87 |
| 5 | human | 3140 | MR1 | major histocompatibility co... | NM_001195000.1 | 59.8% | 59.8% | 327_461del;603_604ins276 |
| 6 | human | 3140 | MR1 | major histocompatibility co... | NM_001195035.1 | 48.8% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaactgatg gcgttcctgt tacctctcat cattgtgtta atggtgaagc 121 acagcgattc ccggacgcac tctctgagat attttcgcct gggcgtttcg gatcccatcc 181 atggggtccc tgaatttatt tcggttgggt acgtggactc gcaccctatc accacatatg 241 acagtgtcac tcggcagaag gagccacggg ccccatggat ggcagagaac ctcgcgcctg 301 atcactggga gaggtacact cagctgctga ggggctggca gcagatgttc aaggtggaac 361 tgaagcgcct acagaggcac tacaatcact cagataatgt ggctcacacc atcaagcagg 421 catgggaggc caatcagcat gagttgctgt atcaaaagaa ttggctggaa gaagaatgta 481 ttgcctggct aaagagattc ctggagtatg ggaaagacac cctacaaaga acagagcccc 541 cactggtcag agtaaatcgc aaagaaactt ttccaggggt tacagctctc ttctgcaaag 601 ctcatggctt ttacccccca gaaatttaca tgacatggat gaaaaacggg gaagaaattg 661 tccaagaaat tgattatgga gacattcttc ccagtgggga tggaacctat caggcgtggg 721 catcaattga gcttgatcct cagagcagca acctttactc ctgtcatgtg gagcactgcg 781 gtgtccacat ggttcttcag gtcccccagg aatcagaaac tatccctctt gtgatgaaag 841 ctgtctctgg gtccattgtc cttgtcattg tgctggctgg agttggtgtt ctagtctgga 901 gaagaaggcc ccgagagcaa aatggagcca tctaccttcc aacaccagat cgatgcccaa 961 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1021 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta