Construct: ORF ccsbBroad304_00795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011460.1_s304c1, BRDN0000408698
- Derived from:
- ccsbBroadEn_00795
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSF2 (3298)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00795
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3298 | HSF2 | heat shock transcription fa... | NM_004506.4 | 100% | 100% | |
| 2 | human | 3298 | HSF2 | heat shock transcription fa... | NM_001135564.1 | 96.6% | 96.6% | 1176_1177ins54 |
| 3 | human | 3298 | HSF2 | heat shock transcription fa... | NM_001243094.2 | 42.7% | 42.5% | 683_684insTCCACACAGTAGGACTG;686_687delTTinsGG;690_691ins901 |
| 4 | mouse | 15500 | Hsf2 | heat shock factor 2 | XM_006512564.2 | 90.8% | 94.7% | (many diffs) |
| 5 | mouse | 15500 | Hsf2 | heat shock factor 2 | NM_008297.3 | 87.5% | 91.6% | (many diffs) |
| 6 | mouse | 15500 | Hsf2 | heat shock factor 2 | XM_006512565.3 | 80.9% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1677
- ORF length:
- 1608
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcagagt tcgaacgtgc cggctttcct cagcaagctg tggacgcttg 121 tggaggaaac ccacactaac gagttcatca cctggagcca gaatggccaa agttttctgg 181 tcttggatga gcaacgattt gcaaaagaaa ttcttcccaa atatttcaag cacaataata 241 tggcaagctt tgtgaggcaa ctgaatatgt atggtttccg taaagtagta catatcgact 301 ctggaattgt aaagcaagaa agagatggtc ctgtagaatt tcagcatcct tacttcaaac 361 aaggacagga tgacttgttg gagaacatta aaaggaaggt ttcatcttca aaaccagaag 421 aaaataaaat tcgtcaggaa gatttaacaa aaattataag tagtgctcag aaggttcaga 481 taaaacagga aactattgag tccaggcttt ctgaattaaa aagtgagaat gagtcccttt 541 ggaaggaggt gtcagaatta cgagcaaagc atgcacaaca gcaacaagtt attcgaaaga 601 ttgtccagtt tattgttaca ttggttcaaa ataaccaact tgtgagttta aaacgtaaaa 661 ggcctctact tctaaacact aatggagccc aaaagaagaa cctgtttcag cacatagtca 721 aagaaccaac tgataatcat catcataaag ttccacacag taggactgaa ggtttaaagc 781 caagggagag gatttcagat gacatcatta tttatgatgt tactgatgat aatgcagatg 841 aagaaaatat cccagttatt ccagaaacta atgaggatgt tatatctgat ccctccaact 901 gtagccagta ccctgatatt gtcatcgttg aagatgacaa tgaagatgag tatgcacctg 961 tcattcagag tggagagcag aatgaaccag ccagagaatc cctaagttca ggcagtgatg 1021 gcagcagccc tctcatgtct agtgctgtcc agctaaatgg ctcatccagt ctgacctcag 1081 aagatccagt gaccatgatg gattccattt tgaatgataa catcaatctt ttgggaaagg 1141 ttgagctgtt ggattatctt gacagtattg actgcagttt agaggacttc caggccatgc 1201 tatcaggaag acaatttagc atagacccag atctcctggt tgatcttttc actagttctg 1261 tgcagatgaa tcccacagat tacatcaata atacaaaatc tgagaataaa ggattagaaa 1321 ctaccaagaa caatgtagtt cagccagttt cggaagaggg aagaaaatct aaatccaaac 1381 cagataagca gcttatccag tataccgcct ttccacttct tgcattcctc gatgggaacc 1441 ctgcttcttc tgttgaacag gcgagtacaa cagcatcatc agaagttttg tcctctgtag 1501 ataaacccat agaagttgat gagcttctgg atagcagcct agacccagaa ccaacccaaa 1561 gtaagcttgt tcgcctggag ccattgactg aagctgaagc tagtgaagct acactgtttt 1621 atttatgtga acttgctcct gcacctctgg atagtgatat gccactttta gatagcttgc 1681 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1741 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1801 tta