Construct: ORF ccsbBroad304_00822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005087.1_s304c1, BRDN0000460415
- Derived from:
- ccsbBroadEn_00822
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA2 (3440)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00822
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 100% | 100% | |
| 2 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 90.2% | 85.7% | (many diffs) |
| 3 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 90.2% | 82% | (many diffs) |
| 4 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 89.5% | 82% | (many diffs) |
| 5 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 89.4% | 83.5% | (many diffs) |
| 6 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 89.2% | 82.1% | (many diffs) |
| 7 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 89.2% | 81.4% | (many diffs) |
| 8 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 88.8% | 80.4% | (many diffs) |
| 9 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 88.7% | 82% | (many diffs) |
| 10 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 88.7% | 81.4% | (many diffs) |
| 11 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 88.3% | 80.9% | (many diffs) |
| 12 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 88% | 80.4% | (many diffs) |
| 13 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 87.4% | 77.7% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 47% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 633
- ORF length:
- 564
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccttgacc tttgctttac tggtggccct cctggtgctc agctgcaagt 121 caagctgctc tgtgggctgt gatctgcctc aaacccacag cctgggtagc aggaggacct 181 tgatgctcct ggcacagatg aggagaatct ctcttttctc ctgcttgaag gacagacatg 241 actttggatt tccccaggag gagtttggca accagttcca aaaggctgaa accatccctg 301 tcctccatga gatgatccag cagatcttca atctcttcag cacaaaggac tcatctgctg 361 cttgggatga gaccctccta gacaaattct acactgaact ctaccagcag ctgaatgacc 421 tggaagcctg tgtgatacag ggggtggggg tgacagagac tcccctgatg aaggaggact 481 ccattctggc tgtgaggaaa tacttccaaa gaatcactct ctatctgaaa gagaagaaat 541 acagcccttg tgcctgggag gttgtcagag cagaaatcat gagatctttt tctttgtcaa 601 caaacttgca agaaagttta agaagtaagg aattgccaac tttcttgtac aaagtggttg 661 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 721 accggtggcg cgttaagtcg acaatcaacc tctggatta