Construct: ORF ccsbBroad304_00824
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010334.1_s304c1, BRDN0000460426
- Derived from:
- ccsbBroadEn_00824
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA5 (3442)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00824
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 100% | 100% | |
| 2 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 91.5% | 86.7% | (many diffs) |
| 3 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 91.3% | 84.6% | (many diffs) |
| 4 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 91% | 86.2% | (many diffs) |
| 5 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 90.1% | 84.1% | (many diffs) |
| 6 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 89.9% | 83.5% | (many diffs) |
| 7 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 89.9% | 82.5% | (many diffs) |
| 8 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 89.8% | 84.2% | (many diffs) |
| 9 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 89.4% | 83.5% | (many diffs) |
| 10 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 89.4% | 82.5% | (many diffs) |
| 11 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 89.2% | 82.5% | (many diffs) |
| 12 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 88.7% | 82% | (many diffs) |
| 13 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 88.3% | 79.8% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 48.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccttgccc tttgttttac tgatggccct ggtggtgctc aactgcaagt 121 caatctgttc tctgggctgt gatctgcctc agacccacag cctgagtaac aggaggactt 181 tgatgataat ggcacaaatg ggaagaatct ctcctttctc ctgcctgaag gacagacatg 241 actttggatt tcctcaggag gagtttgatg gcaaccagtt ccagaaggct caagccatct 301 ctgtcctcca tgagatgatc cagcagacct tcaatctctt cagcacaaag gactcatctg 361 ctacttggga tgagacactt ctagacaaat tctacactga actttaccag cagctgaatg 421 acctggaagc ctgtatgatg caggaggttg gagtggaaga cactcctctg atgaatgtgg 481 actctatcct gactgtgaga aaatactttc aaagaatcac cctctatctg acagagaaga 541 aatacagccc ttgtgcatgg gaggttgtca gagcagaaat catgagatcc ttctctttat 601 cagcaaactt gcaagaaaga ttaaggagga aggaattgcc aactttcttg tacaaagtgg 661 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 721 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta