Construct: ORF ccsbBroad304_00828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014810.1_s304c1, BRDN0000460437
- Derived from:
- ccsbBroadEn_00828
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA14 (3448)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00828
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 100% | 100% | |
| 2 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 91.3% | 84.6% | (many diffs) |
| 3 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 91.1% | 84.1% | (many diffs) |
| 4 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 91.1% | 83% | (many diffs) |
| 5 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 91% | 84.1% | (many diffs) |
| 6 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 91% | 83.5% | (many diffs) |
| 7 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 90.6% | 82% | (many diffs) |
| 8 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 90.2% | 82% | (many diffs) |
| 9 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 90.2% | 82% | (many diffs) |
| 10 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 90.1% | 83% | (many diffs) |
| 11 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 90.1% | 80.9% | (many diffs) |
| 12 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 90% | 82.1% | (many diffs) |
| 13 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 88.5% | 80.9% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 47.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 636
- ORF length:
- 567
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcattgccc tttgctttaa tgatggccct ggtggtgctc agctgcaagt 121 caagctgctc tctgggctgt aatctgtctc aaacccacag cctgaataac aggaggactt 181 tgatgctcat ggcacaaatg aggagaatct ctcctttctc ctgcctgaag gacagacatg 241 actttgaatt tccccaggag gaatttgatg gcaaccagtt ccagaaagct caagccatct 301 ctgtcctcca tgagatgatg cagcagacct tcaatctctt cagcacaaag aactcatctg 361 ctgcttggga tgagaccctc ctagaaaaat tctacattga acttttccag caaatgaatg 421 acctggaagc ctgtgtgata caggaggttg gggtggaaga gactcccctg atgaatgagg 481 actccatcct ggctgtgaag aaatacttcc aaagaatcac tctttatctg atggagaaga 541 aatacagccc ttgtgcctgg gaggttgtca gagcagaaat catgagatcc ctctcttttt 601 caacaaactt gcaaaaaaga ttaaggagga aggatttgcc aactttcttg tacaaagtgg 661 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 721 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta