Construct: ORF ccsbBroad304_00863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009245.1_s304c1, BRDN0000406973
- Derived from:
- ccsbBroadEn_00863
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL15 (3600)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00863
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3600 | IL15 | interleukin 15 | NM_000585.5 | 100% | 100% | |
| 2 | human | 3600 | IL15 | interleukin 15 | NM_172175.3 | 79.1% | 75.5% | (many diffs) |
| 3 | human | 3600 | IL15 | interleukin 15 | NR_037840.3 | 19.5% | 1_863del;1350_2491del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag aatttcgaaa ccacatttga gaagtatttc catccagtgc tacttgtgtt 121 tacttctaaa cagtcatttt ctaactgaag ctggcattca tgtcttcatt ttgggctgtt 181 tcagtgcagg gcttcctaaa acagaagcca actgggtgaa tgtaataagt gatttgaaaa 241 aaattgaaga tcttattcaa tctatgcata ttgatgctac tttatatacg gaaagtgatg 301 ttcaccccag ttgcaaagta acagcaatga agtgctttct cttggagtta caagttattt 361 cacttgagtc cggagatgca agtattcatg atacagtaga aaatctgatc atcctagcaa 421 acaacagttt gtcttctaat gggaatgtaa cagaatctgg atgcaaagaa tgtgaggaac 481 tggaggaaaa aaatattaaa gaatttttgc agagttttgt acatattgtc caaatgttca 541 tcaacacttc ttgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 601 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 661 caatcaacct ctggatta