Construct: ORF ccsbBroad304_00963
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004165.1_s304c1, BRDN0000399101
- Derived from:
- ccsbBroadEn_00963
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMAD5 (4090)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00963
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4090 | SMAD5 | SMAD family member 5 | NM_001001419.3 | 100% | 100% | |
| 2 | human | 4090 | SMAD5 | SMAD family member 5 | NM_001001420.2 | 100% | 100% | |
| 3 | human | 4090 | SMAD5 | SMAD family member 5 | NM_005903.7 | 100% | 100% | |
| 4 | human | 4090 | SMAD5 | SMAD family member 5 | XM_017009470.2 | 100% | 100% | |
| 5 | human | 4090 | SMAD5 | SMAD family member 5 | XM_024446046.1 | 100% | 100% | |
| 6 | human | 4090 | SMAD5 | SMAD family member 5 | XM_024446047.1 | 100% | 100% | |
| 7 | mouse | 17129 | Smad5 | SMAD family member 5 | NM_001164041.1 | 91.6% | 98.9% | (many diffs) |
| 8 | mouse | 17129 | Smad5 | SMAD family member 5 | NM_001164042.1 | 91.6% | 98.9% | (many diffs) |
| 9 | mouse | 17129 | Smad5 | SMAD family member 5 | NM_008541.3 | 91.6% | 98.9% | (many diffs) |
| 10 | mouse | 17129 | Smad5 | SMAD family member 5 | XM_006517117.1 | 91.6% | 98.9% | (many diffs) |
| 11 | mouse | 17129 | Smad5 | SMAD family member 5 | XM_006517118.3 | 91.6% | 98.9% | (many diffs) |
| 12 | mouse | 17129 | Smad5 | SMAD family member 5 | XM_006517119.3 | 58.7% | 63.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac gtcaatggcc agcttgtttt cttttactag tccagcagta aagcgattgt 121 tgggctggaa acaaggtgat gaggaggaga aatgggcaga aaaggcagtt gatgctttgg 181 tgaagaaact aaaaaagaaa aagggtgcca tggaggaact ggagaaagcc ttgagcagtc 241 caggacagcc gagtaaatgt gtcactattc ccagatcttt agatggacgc ctgcaggttt 301 ctcacagaaa aggcttaccc catgttatat attgtcgtgt ttggcgctgg ccggatttgc 361 agagtcatca tgagctaaag ccgttggata tttgtgaatt tccttttgga tctaagcaaa 421 aagaagtttg tatcaaccca taccactata agagagtgga gagtccagtc ttacctccag 481 tattagtgcc tcgtcataat gaattcaatc cacaacacag ccttctggtt cagtttagga 541 acctgagcca caatgaacca cacatgccac aaaatgccac gtttccagat tctttccacc 601 agcccaacaa cactcctttt cccttatctc caaacagccc ttatccccct tctcctgcta 661 gcagcacata tcccaactcc ccagcaagtt ctggaccagg aagtccattt cagctcccag 721 ctgatacgcc tcctcctgcc tatatgccac ctgatgatca gatgggtcaa gataattccc 781 agcctatgga tacaagcaat aatatgattc ctcagattat gcccagtata tccagcaggg 841 atgttcagcc tgttgccTAT GAAGAGCCTA AACATTGGTG TTCAATAGTC TACTATGAAT 901 TAAACAATCG TGTTGGAGAA GCTTTTCATG CATCTTCTAC TAGTGTGTTA GTAGATGGAT 961 TCACAGATCC TTCAAATAAC AAAAGTAGAT TCTGCTTGGG TTTGTTGTCA AATGTTAATC 1021 GTAATTCGAC AATTGAAAAC ACTAGGCGAC ATATTGGAAA AGGTGTTCAT CTGTACTATG 1081 TTGGTGGAGA GGTGTATGCG GAATGCCTCA GTGACAGCAG CATATTTGTA CAGAGTAGGA 1141 ACTGCAACTT TCATCATGGC TTTCATCCCA CCACTGTCTG TAAGATTCCC AGCAGCTGCA 1201 GCCTCAAAAT TTTTAACAAT CAGGAGTTTG CTCAGCTTCT GGCTCAATCT GTCAACCATG 1261 GGTTTGAGGC AGTATATGAG CTCACCAAAA TGTGTACCAT TCGGATGAGT TTTGTCAAGG 1321 GTTGGGGAGC AGAATATCAC CGGCAGGATG TAACCAGCAC CCCATGTTGG ATTGAGATTC 1381 ATCTTCATGG GCCTCTTCAG TGGCTGGATA AAGTCCTTAC TCAGATGGGC TCCCCTCTGA 1441 ACCCCATATC TTCTGTTTCA TACCCAACTT TCTTGTACaa agtggttggt aagcctatcc 1501 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1561 ttaagtcgac aatcaacctc tggatta