Construct: ORF ccsbBroad304_01104
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011302.1_s304c1, BRDN0000461515
- Derived from:
- ccsbBroadEn_01104
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOVA1 (4857)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01104
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_002515.3 | 100% | 100% | |
| 2 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366392.2 | 99.8% | 99.8% | 135_136insGAA |
| 3 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_006489.3 | 95.2% | 95.2% | 447_448ins72 |
| 4 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366393.1 | 92.1% | 91.1% | (many diffs) |
| 5 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366394.1 | 87.4% | 86.3% | (many diffs) |
| 6 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366391.2 | 75.9% | 75.9% | 0_1ins366 |
| 7 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366395.1 | 75.9% | 75.9% | 0_1ins366 |
| 8 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366396.1 | 75.9% | 75.9% | 0_1ins366 |
| 9 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366398.1 | 75.9% | 75.9% | 0_1ins366 |
| 10 | human | 4857 | NOVA1 | NOVA alternative splicing r... | XM_017021345.1 | 75.9% | 75.9% | 0_1ins366 |
| 11 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366390.2 | 71.2% | 71.2% | 0_1ins366;81_82ins72 |
| 12 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_001366397.1 | 71.2% | 71.2% | 0_1ins366;81_82ins72 |
| 13 | human | 4857 | NOVA1 | NOVA alternative splicing r... | NM_006491.2 | 35.1% | 34.5% | (many diffs) |
| 14 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | NM_021361.1 | 96.5% | 99.6% | (many diffs) |
| 15 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_006516150.3 | 91.9% | 94.8% | (many diffs) |
| 16 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_006516151.2 | 89.1% | 90.7% | (many diffs) |
| 17 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_006516152.2 | 88.1% | 90.7% | (many diffs) |
| 18 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_011244160.2 | 88.1% | 90.7% | (many diffs) |
| 19 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_006516153.3 | 73.7% | 75.7% | (many diffs) |
| 20 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_017315157.1 | 73.7% | 75.7% | (many diffs) |
| 21 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_017315158.1 | 73.7% | 75.7% | (many diffs) |
| 22 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_017315159.1 | 69.1% | 71% | (many diffs) |
| 23 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_017315160.1 | 65.6% | 67.2% | (many diffs) |
| 24 | mouse | 664883 | Nova1 | neuro-oncological ventral a... | XM_006516154.3 | 33.3% | 34.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1590
- ORF length:
- 1521
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gatggcggca gctcccatcc agcagaacgg gacccacact ggggttccca 121 tagacctgga cccgccggac tcgcggaaaa ggccgctgga agccccccct gaagccggca 181 gcaccaagag gaccaatacg ggcgaagacg gccagtattt tctaaaggtt ctcataccta 241 gttatgctgc tggatctata attgggaagg gaggacagac aattgttcag ttgcaaaaag 301 aaactggagc caccatcaag ctgtctaagt ccaaagattt ttacccaggt actactgagc 361 gagtgtgctt gatccaggga acggttgaag cactgaatgc agttcatgga ttcattgcag 421 aaaaaattcg agaaatgccc caaaatgtgg ccaagacaga accagtcagc attctacaac 481 cccagaccac cgttaatcca gatcgcatca aacaaacatt gccatcttcc ccaactacca 541 ccaagtcctc tccatctgat cccatgacca cctccagagc taatcaggta aagattatag 601 ttcccaacag cacagcaggt ctgataatag ggaagggagg tgctactgtg aaggctgtaa 661 tggagcagtc aggggcttgg gtgcagcttt cccagaaacc tgatgggatc aacttgcaag 721 agagggttgt cactgtgagt ggagaacctg aacaaaaccg aaaagctgtt gaacttatca 781 tccagaagat acaagaggat ccacaaagtg gcagctgtct caatatcagt tatgccaatg 841 tgacaggtcc agtggcaaat tccaatccaa ccggatctcc ttatgcaaac actgctgaag 901 tgttaccaac tgctgcagca gctgcagggc tattaggaca tgctaacctt gctggcgttg 961 cagcctttcc agcagtttta tctggcttca caggcaatga cctggtggcc atcacctctg 1021 cacttaatac attagccagc tatggatata atctcaacac tttaggttta ggtctcagtc 1081 aagcagcagc aacaggggct ttggctgcag cagctgccag tgccaaccca gcagcagcag 1141 cagccaattt attggccacc tatgccagtg aagcctcagc cagtggcagc acagctggtg 1201 gtacggcggg gacatttgca ttaggtagcc tggctgctgc tactgctgca accaatggat 1261 attttggagc tgcttctccc ctagctgcca gtgccattct aggaacagaa aagtccacag 1321 atggatccaa ggatgtagtt gaaatagcag tgccagaaaa cttagttggt gcaatacttg 1381 gcaaaggagg gaaaacatta gtggaatacc aggagttgac tggtgcaagg atacagatct 1441 ccaaaaaagg agaattcgta cctggcacaa ggaatcggaa ggtaaccatt actggaacac 1501 cagctgcaac acaggctgct caatatttaa ttacacaaag gatcacatat gagcaaggag 1561 ttcgggctgc caatcctcag aaagtgggtt tgccaacttt cttgtacaaa gtggttggta 1621 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1681 ggtggcgcgt taagtcgaca atcaacctct ggatta