Construct: ORF ccsbBroad304_01131
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017984.1_s304c1, BRDN0000410366
- Derived from:
- ccsbBroadEn_01131
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OXCT1 (5019)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01131
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NM_000436.4 | 100% | 100% | |
| 2 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NM_001364301.2 | 99.6% | 99.6% | 725_726insGGTAGA |
| 3 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NM_001364299.2 | 98.6% | 98.6% | 413_433del |
| 4 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NM_001364300.2 | 96.9% | 94.5% | (many diffs) |
| 5 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NM_001364302.2 | 94.2% | 94.2% | 1248_1249ins90 |
| 6 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | XR_001742081.2 | 68.5% | (many diffs) | |
| 7 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NM_001364303.1 | 64.1% | 63.8% | 0_1ins557;2T>A;5_5delTinsGA |
| 8 | human | 5019 | OXCT1 | 3-oxoacid CoA-transferase 1 | NR_157114.2 | 45.1% | 1_67del;1165_1166ins73;1555_3221del | |
| 9 | mouse | 67041 | Oxct1 | 3-oxoacid CoA transferase 1 | NM_024188.6 | 86.6% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1626
- ORF length:
- 1560
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctctcaaa ctcctctcct ccgggcttcg gctctgcgcc tctgcccgcg 121 gatctggggc aacctggtac aagggatgtg tttgttcctt ttccaccagt gctcatcgcc 181 ataccaagtt ttatacagat ccagtagaag ctgtaaaaga catccctgat ggtgccacgg 241 ttttggttgg tggttttggg ctatgtggaa ttccagagaa tcttatagat gctttactga 301 aaactggagt aaaaggacta actgcagtca gcaacaatgc aggggttgac aattttggtt 361 tggggctttt gcttcggtcc aagcagataa aacgcatggt ctcttcatat gtgggagaaa 421 atgcagaatt tgaacgacag tacttatctg gtgaattaga agtggagctg acaccacagg 481 gcacacttgc agagaggatc cgtgcaggcg gggctggagt tcctgcattt tacaccccaa 541 cagggtatgg gaccctggta caagaaggag gatcgcccat caaatacaac aaagatggca 601 gtgttgccat tgccagtaag ccaagagagg tgagggagtt caatggtcag cactttattt 661 tggaggaagc aattacaggg gattttgctt tggtgaaagc ctggaaggcg gaccgagcag 721 gaaacgtgat tttcaggaaa agtgcaagga atttcaactt gccaatgtgc aaagctgcag 781 aaaccacagt ggtagaggtt gaagaaattg tggatattgg agcatttgct ccagaagaca 841 tccatattcc tcagatttat gtacatcgcc ttataaaggg agaaaaatat gagaaaagaa 901 ttgagcgttt atcaatccgg aaagagggag atggggaagc caaatctgct aaacctggag 961 atgacgtaag ggaacgaatc atcaagaggg ccgctcttga gtttgaggat ggcatgtatg 1021 ctaatttggg cataggaatc cctctcctgg ccagcaattt tatcagccca aatataactg 1081 ttcatcttca aagtgaaaat ggagttctgg gtttgggtcc atatccacga caacatgaag 1141 ctgatgcaga tctcatcaat gcaggcaagg aaacagttac tattcttcca ggagcctctt 1201 ttttctccag cgatgaatca tttgcaatga ttagaggtgg acacgtcgat ctgacaatgc 1261 taggagcgat gcaggtttcc aaatatggtg acctggctaa ctggatgata cctgggaaga 1321 tggtgaaagg aatgggaggt gctatggatt tagtgtccag tgcgaaaacc aaagtggtgg 1381 tcaccatgga gcattctgca aagggaaatg cacataaaat catggagaaa tgtacattac 1441 cattgactgg aaagcaatgt gtcaaccgca ttattactga aaaggctgtg tttgatgtgg 1501 acaagaagaa agggttgact ctgattgagc tctgggaagg cctgacagtg gatgacgtac 1561 agaagagtac tgggtgtgat tttgcagttt caccaaaact catgccaatg cagcagatcg 1621 caaattgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1681 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1741 acctctggat ta