Construct: ORF ccsbBroad304_01159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012844.1_s304c1, BRDN0000401352
- Derived from:
- ccsbBroadEn_01159
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDE6G (5148)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01159
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5148 | PDE6G | phosphodiesterase 6G | NM_001365724.1 | 100% | 100% | |
| 2 | human | 5148 | PDE6G | phosphodiesterase 6G | NM_001365725.1 | 100% | 100% | |
| 3 | human | 5148 | PDE6G | phosphodiesterase 6G | NM_002602.4 | 100% | 100% | |
| 4 | human | 5148 | PDE6G | phosphodiesterase 6G | XM_017024736.1 | 100% | 100% | |
| 5 | human | 5148 | PDE6G | phosphodiesterase 6G | XM_006721942.3 | 63.5% | 63.5% | 1_150del |
| 6 | human | 5148 | PDE6G | phosphodiesterase 6G | XM_017024734.1 | 55.4% | 55.4% | 1_210del |
| 7 | human | 5148 | PDE6G | phosphodiesterase 6G | NR_158591.1 | 18.4% | (many diffs) | |
| 8 | human | 5148 | PDE6G | phosphodiesterase 6G | NR_026872.2 | 17.1% | (many diffs) | |
| 9 | mouse | 18588 | Pde6g | phosphodiesterase 6G, cGMP-... | NM_012065.3 | 89.6% | 96.5% | (many diffs) |
| 10 | mouse | 18588 | Pde6g | phosphodiesterase 6G, cGMP-... | XM_006532455.3 | 89.6% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 330
- ORF length:
- 261
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacctggaa ccgcccaagg ctgagttccg gtcagccacc agggtggccg 121 ggggacctgt cacccccagg aaagggcccc ctaaatttaa gcagcgacag accaggcagt 181 tcaagagcaa gcccccaaag aaaggcgttc aagggtttgg ggacgacatc cctggaatgg 241 aaggcctggg aacagacatc acagtcatct gcccttggga ggccttcaac cacctggagc 301 tgcacgagct ggcccaatat ggcatcatct tgccaacttt cttgtacaaa gtggttggta 361 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 421 ggtggcgcgt taagtcgaca atcaacctct ggatta