Construct: ORF ccsbBroad304_01211

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007515.1_s304c1, BRDN0000409539
Derived from:
ccsbBroadEn_01211
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PKM (5315)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01211
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5315 PKM pyruvate kinase M1/2 NM_002654.6 100% 100%
2 human 5315 PKM pyruvate kinase M1/2 XM_005254445.5 100% 100%
3 human 5315 PKM pyruvate kinase M1/2 NM_182470.3 95.8% 95.8% (many diffs)
4 human 5315 PKM pyruvate kinase M1/2 NM_182471.4 95.8% 95.8% (many diffs)
5 human 5315 PKM pyruvate kinase M1/2 XM_017022313.2 95.8% 95.8% (many diffs)
6 human 5315 PKM pyruvate kinase M1/2 NM_001206799.2 95% 94.9% (many diffs)
7 human 5315 PKM pyruvate kinase M1/2 XM_011521670.2 93.8% 93.8% 1_105del
8 human 5315 PKM pyruvate kinase M1/2 NM_001206798.2 91.5% 80.9% 1_47del;200_201ins92
9 human 5315 PKM pyruvate kinase M1/2 NM_001316318.2 90% 89.9% (many diffs)
10 human 5315 PKM pyruvate kinase M1/2 XM_005254443.1 89.8% 89.8% 1_180del
11 human 5315 PKM pyruvate kinase M1/2 XM_006720570.1 87.7% 87.7% 1_222del
12 human 5315 PKM pyruvate kinase M1/2 NM_001206797.2 86% 86% 171_172ins222
13 human 5315 PKM pyruvate kinase M1/2 NM_001206796.3 84.2% 84.1% (many diffs)
14 human 5315 PKM pyruvate kinase M1/2 XR_001751314.2 54.4% 1_139del;1279_1846del;2301_2924del
15 mouse 18746 Pkm pyruvate kinase, muscle NM_011099.3 90.7% 97.7% (many diffs)
16 mouse 18746 Pkm pyruvate kinase, muscle XM_006510855.1 90.7% 97.7% (many diffs)
17 mouse 18746 Pkm pyruvate kinase, muscle NM_001253883.1 86.9% 93.7% (many diffs)
18 mouse 18746 Pkm pyruvate kinase, muscle XM_011242673.1 86.9% 93.7% (many diffs)
19 mouse 18746 Pkm pyruvate kinase, muscle XM_006510856.2 82.9% 84.6% (many diffs)
20 mouse 18746 Pkm pyruvate kinase, muscle XM_011242674.1 81% 87.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1659
ORF length:
1593
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc gaagccccat agtgaagccg ggactgcctt cattcagacc cagcagctgc
121acgcagccat ggctgacaca ttcctggagc acatgtgccg cctggacatt gattcaccac
181ccatcacagc ccggaacact ggcatcatct gtaccattgg cccagcttcc cgatcagtgg
241agacgttgaa ggagatgatt aagtctggaa tgaatgtggc tcgtctgaac ttctctcatg
301gaactcatga gtaccatgcg gagaccatca agaatgtgcg cacagccacg gaaagctttg
361cttctgaccc catcctctac cggcccgttg ctgtggctct agacactaaa ggacctgaga
421tccgaactgg gctcatcaag ggcagcggca ctgcagaggt ggagctgaag aagggagcca
481ctctcaaaat cacgctggat aacgcctaca tggaaaagtg tgacgagaac atcctgtggc
541tggactacaa gaacatctgc aaggtggtgg aagtgggcag caagatctac gtggatgatg
601ggcttatttc tctccaggtg aagcagaaag gtgccgactt cctggtgacg gaggtggaaa
661atggtggctc cttgggcagc aagaagggtg tgaaccttcc tggggctgct gtggacttgc
721ctgctgtgtc ggagaaggac atccaggatc tgaagtttgg ggtcgagcag gatgttgata
781tggtgtttgc gtcattcatc cgcaaggcat ctgatgtcca tgaagttagg aaggtcctgg
841gagagaaggg aaagaacatc aagattatca gcaaaatcga gaatcatgag ggggttcgga
901ggtttgatga aatcctggag gccagtgatg ggatcatggt ggctcgtggt gatctaggca
961ttgagattcc tgcagagaag gtcttccttg ctcagaagat gatgattgga cggtgcaacc
1021gagctgggaa gcctgtcatc tgtgctactc agatgctgga gagcatgatc aagaagcccc
1081gccccactcg ggctgaaggc agtgatgtgg ccaatgcagt cctggatgga gccgactgca
1141tcatgctgtc tggagaaaca gccaaagggg actatcctct ggaggctgtg cgcatgcagc
1201acctgattgc ccgtgaggca gaggctgcca tctaccactt gcaattattt gaggaactcc
1261gccgcctggc gcccattacc agcgacccca cagaagccac cgccgtgggt gccgtggagg
1321cctccttcaa gtgctgcagt ggggccataa tcgtcctcac caagtctggc aggtctgctc
1381accaggtggc cagataccgc ccacgtgccc ccatcattgc tgtgacccgg aatccccaga
1441cagctcgtca ggcccacctg taccgtggca tcttccctgt gctgtgcaag gacccagtcc
1501aggaggcctg ggctgaggac gtggacctcc gggtgaactt tgccatgaat gttggcaagg
1561cccgaggctt cttcaagaag ggagatgtgg tcattgtgct gaccggatgg cgccctggct
1621ccggcttcac caacaccatg cgtgttgttc ctgtgccgtg cccaactttc ttgtacaaag
1681tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1741gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)

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