Construct: ORF ccsbBroad304_01235

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015725.1_s304c1, BRDN0000403237
Derived from:
ccsbBroadEn_01235
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POLD2 (5425)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01235
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5425 POLD2 DNA polymerase delta 2, acc... NM_001127218.2 100% 100%
2 human 5425 POLD2 DNA polymerase delta 2, acc... NM_001256879.1 100% 100%
3 human 5425 POLD2 DNA polymerase delta 2, acc... XM_024446802.1 100% 100%
4 human 5425 POLD2 DNA polymerase delta 2, acc... NM_006230.3 93% 93% 1_105del
5 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956452.1 70.8% 1_110del;1256_1624del;1887_1987del
6 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956453.1 70.7% 1_112del;1258_1626del;1889_1989del
7 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956457.1 68.4% 1_180del;1326_1694del;1957_2057del
8 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956459.1 65% 1_285del;1431_1799del;2062_2162del
9 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956454.1 63.3% 1_343del;1489_1857del;2120_2220del
10 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956458.1 61% 1_429del;1575_1943del;2206_2306del
11 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956456.1 53.8% 1_736del;1882_2250del;2513_2613del
12 human 5425 POLD2 DNA polymerase delta 2, acc... XR_002956455.1 48.7% 1_1011del;2157_2525del;2788_2888del
13 mouse 18972 Pold2 polymerase (DNA directed), ... NM_008894.2 87.9% 94.2% (many diffs)
14 mouse 18972 Pold2 polymerase (DNA directed), ... XM_006514587.3 87.9% 94.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1473
ORF length:
1407
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt ttctgagcag gctgcccaga gggcccacac tctactgtcc ccaccatcag
121ccaacaatgc cacctttgcc cgggtgccag tggcaaccta caccaactcc tcacaaccct
181tccggctagg agagcgcagc tttagccggc agtatgccca catttatgcc acccgcctca
241tccaaatgag acccttcctg gagaaccggg cccagcagca ctggggcagt ggagtgggag
301tgaagaagct gtgtgaactg cagcctgagg agaagtgctg tgtggtgggc actctgttca
361aggccatgcc gctgcagccc tccatcctgc gggaggtcag cgaggagcac aacctgctcc
421cccagcctcc tcggagtaaa tacatacacc cagatgacga gctggtcttg gaagatgaac
481tgcagcgtat caaactaaaa ggcaccattg acgtgtcaaa gctggttacg gggactgtcc
541tggctgtgtt tggctccgtg agagacgacg ggaagtttct ggtggaggac tattgctttg
601ctgaccttgc tccccagaag cccgcacccc cacttgacac agataggttt gtgctactgg
661tgtccggcct gggcctgggt ggcggtggag gcgagagcct gctgggcacc cagctgctgg
721tggatgtggt gacggggcag cttggggacg aaggggagca gtgcagcgcc gcccacgtct
781cccgggttat cctcgctggc aacctcctca gccacagcac ccagagcagg gattctatca
841ataaggccaa atacctcacc aagaaaaccc aggcagccag cgtggaggct gttaagatgc
901tggatgagat cctcctgcag ctgagcgcct cagtgcccgt ggacgtgatg ccaggcgagt
961ttgatcccac caattacacg ctcccccagc agcccctcca cccctgcatg ttcccgctgg
1021ccactgccta ctccacgctc cagctggtca ccaaccccta ccaggccacc attgatggag
1081tcagattttt ggggacatca ggacagaacg tgagtgacat tttccgatac agcagcatgg
1141aggatcactt ggagatcctg gagtggaccc tgcgggtccg tcacatcagc cccacagccc
1201ctgacactct aggttgttac cccttctaca aaactgaccc gttcatcttc ccagagtgcc
1261cgcatgtcta cttttgtggc aacaccccca gctttggctc caaaatcatc cgaggtcctg
1321aggaccagac agtgctgttg gtgactgtcc ctgacttcag tgccacgcag accgcctgcc
1381ttgtgaacct gcgcagcctg gcctgccagc ccatcagctt ctcgggcttc ggggcagagg
1441acgatgacct gggaggcctg gggctgggcc cctgcccaac tttcttgtac aaagtggttg
1501gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1561accggtggcg cgttaagtcg acaatcaacc tctggatta

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