Construct: ORF ccsbBroad304_01254

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011055.1_s304c1, BRDN0000399903
Derived from:
ccsbBroadEn_01254
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PPM1A (5494)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01254
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5494 PPM1A protein phosphatase, Mg2+/M... NM_021003.5 100% 100%
2 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_005267779.1 100% 100%
3 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_005267781.1 100% 100%
4 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536878.1 100% 100%
5 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536879.3 100% 100%
6 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536880.3 100% 100%
7 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536881.3 100% 100%
8 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536882.2 100% 100%
9 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536883.1 100% 100%
10 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021384.1 100% 100%
11 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021385.1 100% 100%
12 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021386.1 100% 100%
13 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021387.1 100% 100%
14 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_024449637.1 100% 100%
15 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021383.1 93.6% 93.6% 1_78del
16 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536872.1 87.6% 87.6% 1_162del
17 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536876.1 87.6% 87.6% 1_162del
18 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_011536877.1 87.6% 87.6% 1_162del
19 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021382.1 87.6% 87.6% 1_162del
20 human 5494 PPM1A protein phosphatase, Mg2+/M... NM_177952.3 83.9% 83.9% 1_219del
21 human 5494 PPM1A protein phosphatase, Mg2+/M... NM_177951.3 83.4% 83% (many diffs)
22 human 5494 PPM1A protein phosphatase, Mg2+/M... XM_017021381.1 83.2% 83.2% 1_231del
23 mouse 19042 Ppm1a protein phosphatase 1A, mag... NM_008910.3 93.5% 98.1% (many diffs)
24 mouse 19042 Ppm1a protein phosphatase 1A, mag... XM_006515603.3 93.5% 98.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg agcattttta gacaagccaa agatggaaaa gcataatgcc caggggcagg
121gtaatgggtt gcgatatggg ctaagcagca tgcaaggctg gcgtgttgaa atggaggatg
181cacatacggc tgtgatcggt ttgccaagtg gacttgaatc gtggtcattc tttgctgtgt
241atgatgggca tgctggttct caggttgcca aatactgctg tgagcatttg ttagatcaca
301tcaccaataa ccaggatttt aaagggtctg caggagcacc ttctgtggaa aatgtaaaga
361atggaatcag aacaggtttt ctggagattg atgaacacat gagagttatg tcagagaaga
421aacatggtgc agatagaagt gggtcaacag ctgtaggtgt cttaatttct ccccaacata
481cttatttcat taactgtgga gactcaagag gtttactttg taggaacagg aaagttcatt
541tcttcacaca agatcacaaa ccaagtaatc cgctggagaa agaacgaatt cagaatgcag
601gtggctctgt aatgattcag cgtgtgaatg gctctctggc tgtatcgagg gcccttgggg
661attttgatta caaatgtgtc catggaaaag gtcctactga gcagcttgtc tcaccagagc
721ctgaagtcca tgatattgaa agatctgaag aagatgatca gttcattatc cttgcatgtg
781atggtatctg ggatgttatg ggaaatgaag agctctgtga ttttgtaaga tccagacttg
841aagtcactga tgaccttgag aaagtttgca atgaagtagt cgacacctgt ttgtataagg
901gaagtcgaga caacatgagt gtgattttga tctgttttcc aaatgcaccc aaagtatcgc
961cagaagcagt gaagaaggag gcagagttgg acaagtacct ggaatgcaga gtagaagaaa
1021tcataaagaa gcagggggaa ggcgtccccg acttagtcca tgtgatgcgc acattagcga
1081gtgagaacat ccccagcctc ccaccagggg gtgaattggc aagcaagagg aatgttattg
1141aagccgttta caatagactg aatccttaca aaaatgacga cactgactct acatcaacag
1201atgatatgtg gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1261tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1321caatcaacct ctggatta

Download FASTA (ORF) (Full)