Construct: ORF ccsbBroad304_01268
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017794.1_s304c1, BRDN0000461529
- Derived from:
- ccsbBroadEn_01268
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP3CB (5532)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01268
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | NM_001142353.2 | 100% | 100% | |
| 2 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | NM_021132.4 | 99.8% | 99.8% | 1185_1186insGTA |
| 3 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | NM_001142354.2 | 98% | 98% | 1368_1369ins30 |
| 4 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | XM_005269944.1 | 97.9% | 97.9% | 1185_1186insGTA;1365_1366ins30 |
| 5 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | NM_001289968.1 | 87.3% | 83.6% | (many diffs) |
| 6 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | XM_017016379.1 | 80.9% | 80.5% | 1272_1273delCCinsGGAGG;1275C>G;1278_1279ins294 |
| 7 | human | 5532 | PPP3CB | protein phosphatase 3 catal... | NM_001289969.1 | 74.1% | 70.4% | (many diffs) |
| 8 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | NM_008914.3 | 95.9% | 99.2% | (many diffs) |
| 9 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | NM_001310426.1 | 95.7% | 99% | (many diffs) |
| 10 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | XM_006518707.1 | 94.3% | 97.5% | (many diffs) |
| 11 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | NM_001310427.1 | 94% | 97.3% | (many diffs) |
| 12 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | XM_006518711.1 | 93.8% | 97.1% | (many diffs) |
| 13 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | XM_006518708.1 | 92.4% | 95.6% | (many diffs) |
| 14 | mouse | 19056 | Ppp3cb | protein phosphatase 3, cata... | XM_017315921.1 | 61.1% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1641
- ORF length:
- 1575
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgccccggag ccggcccggg ctgcaccgcc cccacccccg cccccgccgc 121 cccctcccgg ggctgaccgc gtcgtcaaag ctgtcccttt ccccccaaca catcgcttga 181 catctgaaga agtatttgat ttggatggga tacccagggt tgatgttctg aagaaccact 241 tggtgaaaga aggtcgagta gatgaagaaa ttgcgcttag aattatcaat gagggtgctg 301 ccatccttcg gagagagaaa accatgatag aagtagaagc tccaatcaca gtgtgtggtg 361 acatccatgg ccaatttttt gatctgatga aactttttga agtaggagga tcacctgcta 421 atacacgata cctttttctt ggcgattatg tggacagagg ttattttagt atagagtgtg 481 tcttatattt atgggttctg aagattctat acccaagcac attatttctt ctgagaggca 541 accatgaatg cagacacctt actgaatatt ttacctttaa gcaggaatgt aaaattaagt 601 attcggaaag agtctatgaa gcttgtatgg aagcttttga tagtttgcct cttgctgcac 661 ttttaaacca acagtttctt tgtgttcatg gtggactttc accagaaata cacacactgg 721 atgatattag gagattagat agattcaaag agccacctgc atttggacca atgtgtgact 781 tgttatggtc cgatccttct gaagattttg gaaatgaaaa atcacaggaa cattttagtc 841 acaatacagt tcgaggatgt tcttattttt ataactatcc agcagtgtgt gaatttttgc 901 aaaacaataa tttgttatcg attattagag ctcatgaagc tcaagatgca ggctatagaa 961 tgtacagaaa aagtcaaact acagggttcc cttcattaat aacaattttt tcggcaccta 1021 attacttaga tgtctacaat aataaagctg ctgtattaaa gtatgaaaat aatgtgatga 1081 atattcgaca gtttaactgt tctccacatc cttactggtt gcctaatttt atggatgtct 1141 tcacgtggtc tttaccgttt gttggagaaa aagtgacaga aatgttggta aatgttctga 1201 gtatttgctc tgatgatgaa ctaatgactg aaggtgaaga ccagtttgat gtaggttcag 1261 ctgcagcccg gaaagaaatc ataagaaaca aaattcgagc aattggcaag atggcaagag 1321 tcttctctgt tctcagggag gagagtgaaa gtgtgctgac actcaagggc ctgactccca 1381 cagggatgtt gcctagtgga gtgttagctg gaggacggca gaccctgcaa agtgccacag 1441 ttgaggctat tgaggctgaa aaagcaatac gaggattctc tccaccacat agaatctgca 1501 gttttgaaga ggcaaagggt ttggatagga tcaatgagag aatgccacct cggaaagatg 1561 ctgtacagca agatggtttc aattctctga acaccgcaca tgccactgag aaccacggga 1621 cgggcaacca tactgcccag tgcccaactt tcttgtacaa agtggttggt aagcctatcc 1681 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1741 ttaagtcgac aatcaacctc tggatta