Construct: ORF ccsbBroad304_01292
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014423.1_s304c1, BRDN0000461912
- Derived from:
- ccsbBroadEn_01292
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK2 (5610)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01292
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135651.3 | 100% | 100% | |
| 2 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_002759.3 | 100% | 100% | |
| 3 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_011532987.2 | 100% | 100% | |
| 4 | human | 5610 | EIF2AK2 | eukaryotic translation init... | NM_001135652.2 | 92.5% | 92.5% | 785_786ins123 |
| 5 | human | 5610 | EIF2AK2 | eukaryotic translation init... | XM_017004503.1 | 92.1% | 92.1% | 1246_1247ins129 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1722
- ORF length:
- 1653
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctggtgat ctttcagcag gtttcttcat ggaggaactt aatacatacc 121 gtcagaagca gggagtagta cttaaatatc aagaactgcc taattcagga cctccacatg 181 ataggaggtt tacatttcaa gttataatag atggaagaga atttccagaa ggtgaaggta 241 gatcaaagaa ggaagcaaaa aatgccgcag ccaaattagc tgttgagata cttaataagg 301 aaaagaaggc agttagtcct ttattattga caacaacgaa ttcttcagaa ggattatcca 361 tggggaatta cataggcctt atcaatagaa ttgcccagaa gaaaagacta actgtaaatt 421 atgaacagtg tgcatcgggg gtgcatgggc cagaaggatt tcattataaa tgcaaaatgg 481 gacagaaaga atatagtatt ggtacaggtt ctactaaaca ggaagcaaaa caattggccg 541 ctaaacttgc atatcttcag atattatcag aagaaacctc agtgaaatct gactacctgt 601 cctctggttc ttttgctact acgtgtgagt cccaaagcaa ctctttagtg accagcacac 661 tcgcttctga atcatcatct gaaggtgact tctcagcaga tacatcagag ataaattcta 721 acagtgacag tttaaacagt tcttcgttgc ttatgaatgg tctcagaaat aatcaaagga 781 aggcaaaaag atctttggca cccagatttg accttcctga catgaaagaa acaaagtata 841 ctgtggacaa gaggtttggc atggatttta aagaaataga attaattggc tcaggtggat 901 ttggccaagt tttcaaagca aaacacagaa ttgacggaaa gacttacgtt attaaacgtg 961 ttaaatataa taacgagaag gcggagcgtg aagtaaaagc attggcaaaa cttgatcatg 1021 taaatattgt tcactacaat ggctgttggg atggatttga ttatgatcct gagaccagtg 1081 atgattctct tgagagcagt gattatgatc ctgagaacag caaaaatagt tcaaggtcaa 1141 agactaagtg ccttttcatc caaatggaat tctgtgataa agggaccttg gaacaatgga 1201 ttgaaaaaag aagaggcgag aaactagaca aagttttggc tttggaactc tttgaacaaa 1261 taacaaaagg ggtggattat atacattcaa aaaaattaat tcatagagat cttaagccaa 1321 gtaatatatt cttagtagat acaaaacaag taaagattgg agactttgga cttgtaacat 1381 ctctgaaaaa tgatggaaag cgaacaagga gtaagggaac tttgcgatac atgagcccag 1441 aacagatttc ttcgcaagac tatggaaagg aagtggacct ctacgctttg gggctaattc 1501 ttgctgaact tcttcatgta tgtgacactg cttttgaaac atcaaagttt ttcacagacc 1561 tacgggatgg catcatctca gatatatttg ataaaaaaga aaaaactctt ctacagaaat 1621 tactctcaaa gaaacctgag gatcgaccta acacatctga aatactaagg accttgactg 1681 tgtggaagaa aagcccagag aaaaatgaac gacacacatg tttgccaact ttcttgtaca 1741 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1801 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta