Construct: ORF ccsbBroad304_01383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010333.1_s304c1, BRDN0000405566
- Derived from:
- ccsbBroadEn_01383
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBMS1 (5937)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01383
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5937 | RBMS1 | RNA binding motif single st... | NM_016836.4 | 100% | 100% | |
| 2 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_024453032.1 | 99.7% | 99.7% | 73_74insAGC |
| 3 | human | 5937 | RBMS1 | RNA binding motif single st... | NM_002897.5 | 99.2% | 99.2% | 747_748insGTGAGACTT |
| 4 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_017004626.2 | 99% | 99% | 73_74insAGC;744_745insGTGAGACTT |
| 5 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_005246737.1 | 96.2% | 96.2% | 899_946del |
| 6 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_005246738.3 | 95.9% | 95.9% | 73_74insAGC;896_943del |
| 7 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_005246740.1 | 95.4% | 95.4% | 747_748insGTGAGACTT;890_937del |
| 8 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_024453033.1 | 95.4% | 92.5% | (many diffs) |
| 9 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_024453034.1 | 94.6% | 91.8% | (many diffs) |
| 10 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_005246739.2 | 91.8% | 89.1% | (many diffs) |
| 11 | human | 5937 | RBMS1 | RNA binding motif single st... | XM_005246741.3 | 90.9% | 89.1% | (many diffs) |
| 12 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | NM_020296.2 | 90.2% | 96% | (many diffs) |
| 13 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_006499940.2 | 86.8% | 92.4% | (many diffs) |
| 14 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | NM_001141931.1 | 86.2% | 88.7% | (many diffs) |
| 15 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_030251908.1 | 85.9% | 89.7% | (many diffs) |
| 16 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_030251910.1 | 85.2% | 89.9% | (many diffs) |
| 17 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | NM_001141932.1 | 83% | 85.4% | (many diffs) |
| 18 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_017319146.1 | 82.6% | 86.3% | (many diffs) |
| 19 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_030251909.1 | 82% | 82.6% | (many diffs) |
| 20 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_006499942.3 | 81.9% | 86.4% | (many diffs) |
| 21 | mouse | 56878 | Rbms1 | RNA binding motif, single s... | XM_030251907.1 | 78.9% | 79.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg caaagtgtgg aaacagcaga tgtaccctca gtacgccacc tactattacc 121 cccagtatct gcaagccaag cagtctctgg tcccagccca ccccatggcc cctcccagtc 181 ccagcaccac cagcagtaat aacaacagta gcagcagtag caactcagga tgggatcagc 241 tcagcaaaac gaacctctat atccgaggac tgcctcccca caccaccgac caggacctgg 301 tgaagctctg tcaaccatat gggaaaatag tctccacaaa ggcaattttg gataagacaa 361 cgaacaaatg caaaggttat ggttttgtcg actttgacag ccctgcagca gctcaaaaag 421 ctgtgtctgc cctgaaggcc agtggggttc aagctcaaat ggcaaagcaa caggaacaag 481 atcctaccaa cctctacatt tctaatttgc cactctccat ggatgagcaa gaactagaaa 541 atatgctcaa accatttgga caagttattt ctacaaggat actacgtgat tccagtggta 601 caagtcgtgg tgttggcttt gctaggatgg aatcaacaga aaaatgtgaa gctgttattg 661 gtcattttaa tggaaaattt attaagacac caccaggagt ttctgccccc acagaacctt 721 tattgtgtaa gtttgctgat ggaggacaga aaaagagaca gaacccaaac aaatacatcc 781 ctaatggaag accatggcat agagaaggag aggtgagact tgctggaatg acacttactt 841 acgacccaac tacagctgct atacagaacg gattttatcc ttcaccatac agtattgcta 901 caaaccgaat gatcactcaa acttctatta caccctatat tgcatctcct gtatctgcct 961 accaggtgca aagtccttcg tggatgcaac ctcaaccata tattctacag caccctggtg 1021 ccgtgttaac tccctcaatg gagcacacca tgtcactaca gcccgcatca atgatcagcc 1081 ctctggccca gcagatgagt catctgtcac taggcagcac cggaacatac atgcctgcaa 1141 cgtcagctat gcaaggagcc tacttgccac agtatgcaca tatgcagacg acagcggttc 1201 ctgttgagga ggcaagtggt caacagcagg tggctgtcga gacgtctaat gaccattctc 1261 catatacctt tcaacctaat aagtacccaa ctttcttgta caaagtggtt ggtaagccta 1321 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1381 gcgttaagtc gacaatcaac ctctggatta