Construct: ORF ccsbBroad304_01453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017237.1_s304c1, BRDN0000405991
- Derived from:
- ccsbBroadEn_01453
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS14 (6208)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01453
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6208 | RPS14 | ribosomal protein S14 | NM_001025070.2 | 100% | 100% | |
| 2 | human | 6208 | RPS14 | ribosomal protein S14 | NM_001025071.2 | 100% | 100% | |
| 3 | human | 6208 | RPS14 | ribosomal protein S14 | NM_005617.4 | 100% | 100% | |
| 4 | human | 644068 | RPS14P3 | ribosomal protein S14 pseud... | NR_077246.1 | 50.5% | (many diffs) | |
| 5 | mouse | 20044 | Rps14 | ribosomal protein S14 | NM_020600.4 | 92% | 100% | (many diffs) |
| 6 | mouse | 102634709 | LOC102634709 | 40S ribosomal protein S14 | XR_001781586.1 | 75.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc acctcgaaag gggaaggaaa agaaggaaga acaggtcatc agcctcggac 121 ctcaggtggc tgaaggagag aatgtatttg gtgtctgcca tatctttgca tccttcaatg 181 acacttttgt ccatgtcact gatctttctg gcaaggaaac catctgccgt gtgactggtg 241 ggatgaaggt aaaggcagac cgagatgaat cctcaccata tgctgctatg ttggctgccc 301 aggatgtggc ccagaggtgc aaggagctgg gtatcaccgc cctacacatc aaactccggg 361 ccacaggagg aaataggacc aagacccctg gacctggggc ccagtcggcc ctcagagccc 421 ttgcccgctc gggtatgaag atcgggcgga ttgaggatgt cacccccatc ccctctgaca 481 gcactcgcag gaaggggggt cgccgtggtc gccgtctgtg cccaactttc ttgtacaaag 541 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 601 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta