Construct: ORF ccsbBroad304_01538

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014780.1_s304c1, BRDN0000410590
Derived from:
ccsbBroadEn_01538
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SKP2 (6502)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01538
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6502 SKP2 S-phase kinase associated p... NM_032637.4 100% 100%
2 human 6502 SKP2 S-phase kinase associated p... NM_005983.4 87.5% 82.2% (many diffs)
3 human 6502 SKP2 S-phase kinase associated p... XM_011514082.3 87.1% 86.3% (many diffs)
4 human 6502 SKP2 S-phase kinase associated p... XR_001742203.2 69.9% 1_196del;1258_1470del;1556_1557ins83
5 human 6502 SKP2 S-phase kinase associated p... XM_017009753.1 58.7% 58.7% 0_1ins507
6 human 6502 SKP2 S-phase kinase associated p... XM_011514083.3 49.2% 44.3% (many diffs)
7 human 6502 SKP2 S-phase kinase associated p... NM_001243120.1 38.9% 34.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1296
ORF length:
1230
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgca caggaagcac ctccaggaga ttccagacct gagtagcaac gttgccacca
121gcttcacgtg gggatgggat tccagcaaga cttctgaact gctgtcaggc atgggggtct
181ccgccctgga gaaagaggag cccgacagtg agaacatccc ccaggaactg ctctcaaacc
241tgggccaccc ggagagcccc ccacggaaac ggctgaagag caaagggagt gacaaagact
301ttgtgattgt ccgcaggcct aagctaaatc gagagaactt tccaggtgtt tcatgggact
361cccttccgga tgagctgctc ttgggaatct tttcctgtct gtgcctccct gagctgctaa
421aggtctctgg tgtttgtaag aggtggtatc gcctagcgtc tgatgagtct ctatggcaga
481ccttagacct cacaggtaaa aatctgcacc cggatgtgac tggtcggttg ctgtctcaag
541gggtgattgc cttccgctgc ccacgatcat ttatggacca accattggct gaacatttca
601gcccttttcg tgtacagcac atggacctat cgaactcagt tatagaagtg tccaccctcc
661acggcatact gtctcagtgt tccaagttgc agaatctaag cctggaaggc ctgcggcttt
721cggatcccat tgtcaatact ctcgcaaaaa actcaaattt agtgcgactt aacctttctg
781ggtgttctgg attctctgaa tttgccctgc agactttgct aagcagctgt tccagactgg
841atgagctgaa cctctcctgg tgttttgatt tcactgaaaa gcatgtacag gtggctgttg
901cgcatgtgtc agagaccatc acccagctga atcttagcgg ctacagaaag aatctccaga
961aatcagatct ctctacttta gttagaagat gccccaatct tgtccatcta gacttaagtg
1021atagtgtcat gctaaagaat gactgctttc aggaattttt ccagctcaac tacctccaac
1081acctatcact cagtcggtgc tatgatataa tacctgaaac tttactatta gtgacaagag
1141ctggggttag gatccggttg gactctgaca tcggatgccc tcaaacatac agaacttcca
1201aactcaagtc cagccataag ctattttgcc aacatgtcag agtaatctgt atttttgtat
1261gtgatttcta cttttataga cttgttttaa aacaataccc aactttcttg tacaaagtgg
1321ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1381ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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