Construct: ORF ccsbBroad304_01565
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004072.1_s304c1, BRDN0000407594
- Derived from:
- ccsbBroadEn_01565
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FSCN1 (6624)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01565
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6624 | FSCN1 | fascin actin-bundling prote... | NM_003088.4 | 100% | 100% | |
| 2 | mouse | 14086 | Fscn1 | fascin actin-bundling prote... | NM_007984.2 | 90.3% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac cgccaacggc acagccgagg cggtgcagat ccagttcggc ctcatcaact 121 gcggcaacaa gtacctgacg gccgaggcgt tcgggttcaa ggtgaacgcg tccgccagca 181 gcctgaagaa gaagcagatc tggacgctgg agcagccccc tgacgaggcg ggcagcgcgg 241 ccgtgtgcct gcgcagccac ctgggccgct acctggcggc ggacaaggac ggcaacgtga 301 cctgcgagcg cgaggtgccc ggtcccgact gccgtttcct catcgtggcg cacgacgacg 361 gtcgctggtc gctgcagtcc gaggcgcacc ggcgctactt cggcggcacc gaggaccgcc 421 tgtcctgctt cgcgcagacg gtgtcccccg ccgagaagtg gagcgtgcac atcgccatgc 481 accctcaggt caacatctac agcgtcaccc gtaagcgcta cgcgcacctg agcgcgcggc 541 cggccgacga gatcgccgtg gaccgcgacg tgccctgggg cgtcgactcg ctcatcaccc 601 tcgccttcca ggaccagcgc tacagcgtgc agaccgccga ccaccgcttc ctgcgccacg 661 acgggcgcct ggtggcgcgc cccgagccgg ccactggcta cacgctggag ttccgctccg 721 gcaaggtggc cttccgcgac tgcgagggcc gttacctggc gccgtcgggg cccagcggca 781 cgctcaaggc gggcaaggcc accaaggtgg gcaaggacga gctctttgct ctggagcaga 841 gctgcgccca ggtcgtgctg caggcggcca acgagaggaa cgtgtccacg cgccagggta 901 tggacctgtc tgccaatcag gacgaggaga ccgaccagga gaccttccag ctggagatcg 961 accgcgacac caaaaagtgt gccttccgta cccacacggg caagtactgg acgctgacgg 1021 ccaccggggg cgtgcagtcc accgcctcca gcaagaatgc cagctgctac tttgacatcg 1081 agtggcgtga ccggcgcatc acactgaggg cgtccaatgg caagtttgtg acctccaaga 1141 agaatgggca gctggccgcc tcggtggaga cagcagggga ctcagagctc ttcctcatga 1201 agctcatcaa ccgccccatc atcgtgttcc gcggggagca tggcttcatc ggctgccgca 1261 aggtcacggg caccctggac gccaaccgct ccagctatga cgtcttccag ctggagttca 1321 acgatggcgc ctacaacatc aaagactcca caggcaaata ctggacggtg ggcagtgact 1381 ccgcggtcac cagcagcggc gacactcctg tggacttctt cttcgagttc tgcgactata 1441 acaaggtggc catcaaggtg ggcgggcgct acctgaaggg cgaccacgca ggcgtcctga 1501 aggcctcggc ggaaaccgtg gaccccgcct cgctctggga gtactaccca actttcttgt 1561 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1621 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a