Construct: ORF ccsbBroad304_01590

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009278.1_s304c1, BRDN0000405537
Derived from:
ccsbBroadEn_01590
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SRD5A2 (6716)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01590
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6716 SRD5A2 steroid 5 alpha-reductase 2 NM_000348.4 100% 100%
2 human 6716 SRD5A2 steroid 5 alpha-reductase 2 XM_011533069.2 69% 63.9% (many diffs)
3 human 6716 SRD5A2 steroid 5 alpha-reductase 2 XM_011533072.2 65.7% 64.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
831
ORF length:
762
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcaggttcag tgccagcaga gcccagtgct ggcaggcagc gccactttgg
121tcgcccttgg ggcactggcc ttgtacgtcg cgaagccctc cggctacggg aagcacacgg
181agagcctgaa gccggcggct acccgcctgc cagcccgcgc cgcctggttc ctgcaggagc
241tgccttcctt cgcggtgccc gcggggatcc tcgcccggca gcccctctcc ctcttcgggc
301cacctgggac ggtacttctg ggcctcttct gcctacatta cttccacagg acatttgtgt
361actcactgct caatcgaggg aggccttatc cagctatact cattctcaga ggcactgcct
421tctgcactgg aaatggagtc cttcaaggct actatctgat ttactgtgct gaataccctg
481atgggtggta cacagacata cggtttagct tgggtgtctt cttatttatt ttgggaatgg
541gaataaacat tcatagtgac tatatattgc gccagctcag gaagcctgga gaaatcagct
601acaggattcc acaaggtggc ttgtttacgt atgtttctgg agccaatttc ctcggtgaga
661tcattgaatg gatcggctat gccctggcca cttggtccct cccagcactt gcatttgcat
721ttttctcact ttgtttcctt gggctgcgag cttttcacca ccataggttc tacctcaaga
781tgtttgagga ctaccccaaa tctcggaaag cccttattcc attcatcttt ttgccaactt
841tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
901agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)