Construct: ORF ccsbBroad304_01779

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016857.1_s304c1, BRDN0000407676
Derived from:
ccsbBroadEn_01779
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WNT5A (7474)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01779
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7474 WNT5A Wnt family member 5A NM_003392.4 100% 100%
2 human 7474 WNT5A Wnt family member 5A XM_017007127.1 96.1% 95.9% 1_43del;44_45insT;46G>A
3 human 7474 WNT5A Wnt family member 5A NM_001256105.1 96% 96% 0_1ins45
4 human 7474 WNT5A Wnt family member 5A XM_006713324.1 96% 96% 0_1ins45
5 human 7474 WNT5A Wnt family member 5A XM_011534085.2 96% 96% 0_1ins45
6 human 7474 WNT5A Wnt family member 5A XM_011534086.2 96% 96% 0_1ins45
7 human 7474 WNT5A Wnt family member 5A XM_011534087.2 96% 96% 0_1ins45
8 human 7474 WNT5A Wnt family member 5A XM_011534088.2 96% 96% 0_1ins45
9 human 7474 WNT5A Wnt family member 5A XM_011534089.1 96% 96% 0_1ins45
10 human 7474 WNT5A Wnt family member 5A XM_017007128.1 96% 96% 0_1ins45
11 mouse 22418 Wnt5a wingless-type MMTV integrat... NM_009524.3 90% 98.6% (many diffs)
12 mouse 22418 Wnt5a wingless-type MMTV integrat... XM_006518926.3 87.3% 95.3% (many diffs)
13 mouse 22418 Wnt5a wingless-type MMTV integrat... XM_006518923.3 86.9% 94.9% (many diffs)
14 mouse 22418 Wnt5a wingless-type MMTV integrat... NM_001256224.1 85.4% 94.2% (many diffs)
15 mouse 22418 Wnt5a wingless-type MMTV integrat... XM_006518924.2 85.4% 94.2% (many diffs)
16 mouse 22418 Wnt5a wingless-type MMTV integrat... XM_006518925.3 85.4% 94.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1206
ORF length:
1140
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gaagtccatt ggaatattaa gcccaggagt tgctttgggg atggctggaa
121gtgcaatgtc ttccaagttc ttcctagtgg ctttggccat atttttctcc ttcgcccagg
181ttgtaattga agccaattct tggtggtcgc taggtatgaa taaccctgtt cagatgtcag
241aagtatatat tataggagca cagcctctct gcagccaact ggcaggactt tctcaaggac
301agaagaaact gtgccacttg tatcaggacc acatgcagta catcggagaa ggcgcgaaga
361caggcatcaa agaatgccag tatcaattcc gacatcgaag gtggaactgc agcactgtgg
421ataacacctc tgtttttggc agggtgatgc agataggcag ccgcgagacg gccttcacat
481acgcggtgag cgcagcaggg gtggtgaacg ccatgagccg ggcgtgccgc gagggcgagc
541tgtccaccTG CGGCTGCAGC CGCGCCGCGC GCCCCAAGGA CCTGCCGCGG GACTGGCTCT
601GGGGCGGCTG CGGCGACAAC ATCGACTATG GCTACCGCTT TGCCAAGGAG TTCGTGGACG
661CCCGCGAGCG GGAGCGCATC CACGCCAAGG GCTCCTACGA GAGTGCTCGC ATCCTCATGA
721ACCTGCACAA CAACGAGGCC GGCCGCAGGA CGGTGTACAA CCTGGCTGAT GTGGCCTGCA
781AGTGCCATGG GGTGTCCGGC TCATGTAGCC TGAAGACATG CTGGCTGCAG CTGGCAGACT
841TCCGCAAGGT GGGTGATGCC CTGAAGGAGA AGTACGACAG CGCGGCGGCC ATGCGGCTCA
901ACAGCCGGGG CAAGTTGGTA CAGGTCAACA GCCGCTTCAA CTCGCCCACC ACACAAGACC
961TGGTCTACAT CGACCCCAGC CCTGACTACT GCGTGCGCAA TGAGAGCACC GGCTCGCTGG
1021GCACGCAGGG CCGCCTGTGC AACAAGACGT CGGAGGGCAT GGATGGCTGC GAGCTCATGT
1081GCTGCGGCCG TGGCTACGAC CAGTTCAAGA CCGTGCAGAC GGAGCGCTGC CACTGCAAGT
1141TCCACTGGTG CTGCTACGTC AAGTGCAAGA AGTGCACGGA GATCGTGGAC CAGTTTGTGT
1201GCAAGTACCC AACTTTCTTG TACaaagtgg ttggtaagcc tatccctaac cctctcctcg
1261gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1321acctctggat ta

Download FASTA (ORF) (Full)

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