Construct: ORF ccsbBroad304_01876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010913.1_s304c1, BRDN0000399209
- Derived from:
- ccsbBroadEn_01876
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBM10 (8241)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01876
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_005676.5 | 100% | 100% | |
| 2 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_001204467.2 | 99.8% | 99.8% | 1059_1060insGTG |
| 3 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452459.1 | 96.4% | 96.4% | 1572_1673del |
| 4 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452460.1 | 96.3% | 96.3% | 1059_1060insGTG;1569_1670del |
| 5 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_001204468.1 | 93.4% | 93.4% | 1_195del |
| 6 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_005272677.4 | 93.3% | 93.3% | 1_195del;1254_1255insGTG |
| 7 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_001204466.2 | 91.7% | 91.7% | 201_202ins231 |
| 8 | human | 8241 | RBM10 | RNA binding motif protein 10 | NM_152856.3 | 91.6% | 91.6% | 201_202ins231;828_829insGTG |
| 9 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452457.1 | 90.3% | 90.3% | 1_195del;1767_1868del |
| 10 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452458.1 | 90.2% | 90.2% | 1_195del;1254_1255insGTG;1764_1865del |
| 11 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452462.1 | 88.4% | 88.4% | 201_202ins231;1341_1442del |
| 12 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_005272678.4 | 85.7% | 85.7% | 1_195del;396_397ins231 |
| 13 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_005272679.4 | 85.6% | 85.6% | 1_195del;396_397ins231;1023_1024insGTG |
| 14 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452461.1 | 82.8% | 82.8% | 1_195del;396_397ins231;1536_1637del |
| 15 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_017029884.2 | 78.1% | 78.1% | 0_1ins609 |
| 16 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_017029885.1 | 78% | 78% | 0_1ins609;450_451insGTG |
| 17 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452463.1 | 75.4% | 75.4% | 0_1ins609;963_1064del |
| 18 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452464.1 | 75.4% | 75.4% | 0_1ins609;963_1064del |
| 19 | human | 8241 | RBM10 | RNA binding motif protein 10 | XM_024452465.1 | 64.1% | 64.1% | 0_1ins936;636_737del |
| 20 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | NM_145627.2 | 90% | 96% | (many diffs) |
| 21 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | NM_001167775.1 | 89.9% | 95.9% | (many diffs) |
| 22 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527618.3 | 89.9% | 95.9% | (many diffs) |
| 23 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | NM_001167776.1 | 81.9% | 87.9% | (many diffs) |
| 24 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527619.3 | 81.8% | 87.8% | (many diffs) |
| 25 | mouse | 433287 | Gm15455 | predicted gene 15455 | NM_001161816.1 | 81.1% | 85.1% | (many diffs) |
| 26 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527620.3 | 73.9% | 79.1% | (many diffs) |
| 27 | mouse | 236732 | Rbm10 | RNA binding motif protein 10 | XM_006527621.3 | 69.5% | 74.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2856
- ORF length:
- 2790
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gtatgaaaga cgtggtggtc gtggtgacag gactggccgc tatggagcca 121 ctgaccgctc gcaggatgat ggtggggaga accgcagccg agaccacgac taccgggaca 181 tggactaccg ttcatatcct cgcgagtatg gcagccagga gggcaagcat gactatgacg 241 actcatctga ggagcagagt gcggaggatt cctacgaggc ctccccgggc tccgagactc 301 agcgtaggcg gcggcggcgg cacaggcaca gccccaccgg cccgccaggc ttcccccgag 361 acggcgacta tcgggaccag gactatcgga ccgagcaagg ggaggaggag gaggaggagg 421 aggatgagga ggaggaggag aaggccagta acatcgtcat gctgaggatg ctgccacagg 481 cagccactga ggatgacatc cgtggccagc tgcagtcgca cggcgtgcaa gcacgggagg 541 ttcggctgat gcggaacaaa tcttcaggtc agagccgggg cttcgccttc gtcgagttta 601 gtcacttgca ggacgctaca cgatggatgg aagccaatca gcactccctc aacatcctgg 661 gccagaaggt gtcgatgcac tacagtgacc ccaagcccaa gatcaatgag gactggctgt 721 gcaataagtg tggcgtccag aacttcaaac gccgagagaa gtgcttcaaa tgtggcgtgc 781 ccaagtcaga ggcagagcag aagctgcccc tcggcacgag gctggatcag cagacactgc 841 cactgggtgg ccgggagctg agccagggcc tgcttcccct gccgcagccc taccaggccc 901 agggagtcct ggcctcccaa gccctgtcac agggctcgga gccaagctca gagaacgcca 961 atgacaccat cattttgcgc aacctgaacc cacacagcac catggattcc atcctggggg 1021 ccctggcacc ctacgcggtg ctgtcctcct ccaacgtgcg cgtcataaag gacaagcaga 1081 cccaactgaa ccgcggcttt gccttcatcc agctctccac catcgtggag gcagcccagc 1141 tgctgcagat cctgcaggcc ctgcacccac cactcactat cgacggcaag accatcaatg 1201 ttgagtttgc caagggttct aagagggaca tggcctccaa tgaaggcagt cgcatcagtg 1261 ctgcctctgt ggccagcact gccattgctg cggcccagtg ggccatctca caggcctccc 1321 aaggtgggga gggtacctgg gccacctccg aggagccgcc ggtcgactac agctactacc 1381 aacaggatga gggctatggc aacagccagg gcacagagtc ttccctctat gcccatggct 1441 acctcaaggg caccaagggc cctggcatca ctggaaccaa aggggatccc actggagcag 1501 gtcccgaggc ctccctagag cctggggccg actctgtgtc gatgcaggct ttctctcgcg 1561 cccagcctgg tgctgctcct ggcatctacc aacaatcagc cgaggcgagc agtagccagg 1621 gcactgctgc caacagccag tcgtatacca tcatgtcacc cgctgtgctc aaatctgagc 1681 tccagagccc tacccatcct agttctgctc tcccaccggc taccagcccc actgcccagg 1741 aatcctacag ccagtaccct gttcccgacg tctctaccta ccagtacgat gagacctccg 1801 gctactacta tgacccccag accggcctct actatgaccc caactcccag tattactaca 1861 atgctcagag ccagcagtac ctgtactggg atggggagag gcggacctat gttcccgccc 1921 tggagcagtc ggccgacgga cataaggaga caggggcacc ctcgaaggag ggcaaagaga 1981 agaaggagaa gcacaagacc aagacagctc aacagattgc caaggacatg gaacgctggg 2041 cccgcagtct caacaaacaa aaagaaaact tcaaaaatag cttccagcct atcagctccc 2101 tgcgagatga cgagaggcgg gagtcagcca ctgcagatgc tggctatgcc atcctcgaga 2161 agaagggagc actagccgag agacagcaca ccagcatgga tctcccgaaa ttggccagtg 2221 acgaccgccc aagccctccg cgaggactgg tggcagccta cagcggggag agtgacagtg 2281 aggaggagca ggagcgtggg ggccctgagc gggaggagaa gctcaccgac tggcagaagc 2341 tggcctgtct gctctgccga cgccagttcc ccagcaaaga ggcgctcatc cggcaccagc 2401 agctctcagg gctccacaag caaaaccttg agattcaccg gcgagcccac ttgtcagaaa 2461 acgagctaga agcactagag aagaatgaca tggagcaaat gaagtaccgg gaccgtgcag 2521 ctgaacgcag agaaaagtat ggcatccccg agccgccaga gcccaagagg aggaagtacg 2581 gcggcatatc cacagcctct gtagacttcg agcagcctac tcgggacggg ctgggcagtg 2641 acaacattgg cagtcggatg ctgcaggcca tgggctggaa agagggcagc ggcctgggcc 2701 gcaagaagca gggcattgta acgcctatcg aggcccaaac acgggtgcgg ggctccggcc 2761 tgggtgcacg gggcagctcc tacggggtca cctcaaccga gtcctacaag gagacactgc 2821 acaagacaat ggtgacccgc ttcaacgagg cccagtgccc aactttcttg tacaaagtgg 2881 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2941 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta