Construct: ORF ccsbBroad304_02049
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010955.1_s304c1, BRDN0000402640
- Derived from:
- ccsbBroadEn_02049
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTL8C (8933)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02049
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8933 | RTL8C | retrotransposon Gag like 8C | NM_001078171.2 | 100% | 100% | |
| 2 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001078172.1 | 95.2% | 93.8% | (many diffs) |
| 3 | human | 441518 | RTL8B | retrotransposon Gag like 8B | NM_001078173.2 | 94.3% | 92.9% | (many diffs) |
| 4 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001134321.2 | 59.2% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cggtcgggtg cagctgataa aggccctcct ggccttgccg atccggcctg 121 cgacgcgtcg ctggaggaac ccgattccct ttcccgagac gtttgacggc gataccgacc 181 gactcccgga gttcatcgtg cagacgggct cctacatgtt cgtggacgag aacacgttct 241 ccagcgacgc cctgaaggtg acgttcctca tcacccgcct cacagggccc gccctgcagt 301 gggtgatccc ctacatcaag aaggagagcc ccctcctcaa tgattaccgg ggctttctgg 361 ccgagatgaa gcgagtcttt ggatgggagg aggacgagga cttctaccca actttcttgt 421 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 481 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a