Construct: ORF ccsbBroad304_02064
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017619.1_s304c1, BRDN0000400609
- Derived from:
- ccsbBroadEn_02064
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOCS3 (9021)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02064
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9021 | SOCS3 | suppressor of cytokine sign... | NM_003955.4 | 100% | 100% | |
| 2 | mouse | 12702 | Socs3 | suppressor of cytokine sign... | NM_007707.3 | 90.8% | 96.8% | (many diffs) |
| 3 | mouse | 12702 | Socs3 | suppressor of cytokine sign... | XM_011248707.2 | 90.8% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt cacccacagc aagtttcccg ccgccgggat gagccgcccc ctggacacca 121 gcctgcgcct caagaccttc agctccaaga gcgagtacca gctggtggtg aacgcagtgc 181 gcaagctgca ggagagcggc ttctactgga gcgcagtgac cggcggcgag gcgaaccTGC 241 TGCTCAGTGC CGAGCCCGCC GGCACCTTTC TGATCCGCGA CAGCTCGGAC CAGCGCCACT 301 TCTTCACGCT CAGCGTCAAG ACCCAGTCTG GGACCAAGAA CCTGCGCATC CAGTGTGAGG 361 GGGGCAGCTT CTCTCTGCAG AGCGATCCCC GGAGCACGCA GCCCGTGCCC CGCTTCGACT 421 GCGTGCTCAA GCTGGTGCAC CACTACATGC CGCCCCCTGG AGCCCCCTCC TTCCCCTCGC 481 CACCTACTGA ACCCTCCTCC GAGGTGCCCG AGCAGCCGTC TGCCCAGCCA CTCCCTGGGA 541 GTCCCCCCAG AAGAGCCTAT TACATCTACT CCGGGGGCGA GAAGATCCCC CTGGTGTTGA 601 GCCGGCCCCT CTCCTCCAAC GTGGCCACTC TTCAGCATCT CTGTCGGAAG ACCGTCAACG 661 GCCACCTGGA CTCCTATGAG AAAGTCACCC AGCTGCCGGG GCCCATTCGG GAGTTCCTGG 721 ACCAGTACGA TGCCCCGCTT TACCCAACTT TCTTGTACaa agtggttggt aagcctatcc 781 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 841 ttaagtcgac aatcaacctc tggatta