Construct: ORF ccsbBroad304_02122

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008583.1_s304c1, BRDN0000411833
Derived from:
ccsbBroadEn_02122
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GCM2 (9247)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02122
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9247 GCM2 glial cells missing transcr... NM_004752.4 100% 100%
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1587
ORF length:
1518
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gccggcggcc gcggtgcagg aagcggtcgg cgtgtgctcc tacgggatgc
121agctcagctg ggacatcaac gatccgcaga tgcctcagga gctggccctc tttgaccaat
181tccgagagtg gcctgacggc tatgtgcgct tcatctacag cagcgatgag aagaaggcac
241agcgtcacct gagcggctgg gccatgcgca acaccaacaa ccacaatggc cacatcctca
301agaagtcgtg cctgggtgtg gtggtgtgta cacaggcctg caccctgccc gacggttccc
361gcctgcagct gaggccggcc atctgcgaca aggcacggct gaaacagcag aagaaggcat
421gccctaactg tcattctgct ttggagttga ttccttgtcg agggcacagc ggataccccg
481taaccaactt ttggcggctt gatggcaacg cgatcttttt tcaggccaag ggagttcatg
541atcatccaag accagagagc aaatcagaga cagaagctag aagaagcgcc atcaagagac
601aaatggcctc tttctaccaa ccccagaaaa agagaattcg agaatccgag gcagaagaaa
661atcaagacag cagtggtcat ttcagcaaca tacctccctt ggaaaatcca gaagactttg
721atatagttac tgaaaccagc ttccctattc cagggcagcc ttgcccttcc ttcccaaagt
781ctgatgttta caaagctacc tgtgacctag ccacctttca aggagacaaa atgccaccct
841tccagaaata ctcaagccca agaatctatt tgcctaggcc accttgcagc tatgaattgg
901caaaccctgg ttatacaaat tcaagcccat atcccaccct ttataaggat tccaccagta
961tccctaatga cacagactgg gttcatctga acacactaca atgtaatgtc aattcataca
1021gcagctatga gagaagcttt gatttcacca acaaacagca tggctggaaa ccagctcttg
1081gaaaacccag ccttgtggaa aggactaacc atgggcagtt tcaggccatg gccactcgcc
1141cttattataa cccagagctt ccctgcaggt acctcacgac tccaccacca ggtgcccctg
1201ccctacaaac cgtgatcacc accaccacta aagtgtccta ccaggcctac cagccccctg
1261ctatgaaata cagtgacagt gtgcgagagg tgaagagcct ttcgagctgt aactatgctc
1321ctgaagatac tgggatgtct gtctatccag aaccctgggg tcctccggtg acagtcacca
1381gggcagcctc tccttcaggg ccacctccta tgaaaattgc aggagattgc cgggccatca
1441gacccactgt ggctattccc cacgagccag tttcctctag gacagatgaa gcagagactt
1501gggatgtgtg tctgtctggg ctgggctccg cagtcagtta ctcagacaga gtgggtccct
1561tctttaccta caacaatgag gattttttgc caactttctt gtacaaagtg gttggtaagc
1621ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1681ggcgcgttaa gtcgacaatc aacctctgga tta

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