Transcript: Human NM_004752.4

Homo sapiens glial cells missing transcription factor 2 (GCM2), mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
GCM2 (9247)
Length:
2541
CDS:
249..1769

Additional Resources:

NCBI RefSeq record:
NM_004752.4
NBCI Gene record:
GCM2 (9247)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004752.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000426185 GCAAACTGTAGGAGGTTAAAT pLKO_005 2030 3UTR 100% 15.000 21.000 N GCM2 n/a
2 TRCN0000414101 TAAGTTCACTTAGCTTATTAG pLKO_005 2058 3UTR 100% 13.200 9.240 N GCM2 n/a
3 TRCN0000417225 TCAAGCCCAAGAATCTATTTG pLKO_005 1032 CDS 100% 13.200 9.240 N GCM2 n/a
4 TRCN0000004953 CCAGAAGACTTTGATATAGTT pLKO.1 888 CDS 100% 5.625 3.938 N GCM2 n/a
5 TRCN0000004952 CATGATAGTTTCCTAACCTTT pLKO.1 2375 3UTR 100% 4.950 3.465 N GCM2 n/a
6 TRCN0000004955 CCTTATTATAACCCAGAGCTT pLKO.1 1320 CDS 100% 2.640 1.848 N GCM2 n/a
7 TRCN0000004954 GCCCTAACTGTCATTCTGCTT pLKO.1 601 CDS 100% 2.640 1.848 N GCM2 n/a
8 TRCN0000004956 CAAGAGACAAATGGCCTCTTT pLKO.1 773 CDS 100% 0.495 0.347 N GCM2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004752.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02122 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02122 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466944 TATCTTCATCGTTGTCGTTGCAGG pLX_317 21.9% 100% 100% V5 n/a
Download CSV