Construct: ORF ccsbBroad304_02137

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004836.1_s304c1, BRDN0000403163
Derived from:
ccsbBroadEn_02137
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TRIP13 (9319)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02137
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9319 TRIP13 thyroid hormone receptor in... NM_004237.4 100% 100%
2 human 9319 TRIP13 thyroid hormone receptor in... XM_011514163.2 100% 100%
3 human 9319 TRIP13 thyroid hormone receptor in... NM_001166260.2 66.8% 66.8% 867_868ins429
4 mouse 69716 Trip13 thyroid hormone receptor in... NM_027182.2 87.5% 93.2% (many diffs)
5 mouse 69716 Trip13 thyroid hormone receptor in... XR_873456.2 48.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1362
ORF length:
1296
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga cgaggccgtg ggcgacctga agcaggcgct tccctgtgtg gccgagtcgc
121caacggtcca cgtggaggtg catcagcgcg gcagcagcac tgcaaagaaa gaagacataa
181acctgagtgt tagaaagcta ctcaacagac ataatattgt gtttggtgat tacacatgga
241ctgagtttga tgaacctttt ttgaccagaa atgtgcagtc tgtgtctatt attgacacag
301aattaaaggt taaagactca cagcccatcg atttgagtgc atgcactgtt gcacttcaca
361ttttccagct gaatgaagat ggccccagca gtgaaaatct ggaggaagag acagaaaaca
421taattgcagc aaatcactgg gttctacctg cagctgaatt ccatgggctt tgggacagct
481tggtatacga tgtggaagtc aaatcccatc tcctcgatta tgtgatgaca actttactgt
541tttcagacaa gaacgtcaac agcaacctca tcacctggaa ccgggtggtg ctgctccacg
601gtcctcctgg cactggaaaa acatccctgt gtaaagcgtt agcccagaaa ttgacaatta
661gactttcaag caggtaccga tatggccaat taattgaaat aaacagccac agcctctttt
721ctaagtggtt ttcggaaagt ggcaagctgg taaccaagat gtttcagaag attcaggatt
781tgattgatga taaagacgcc ctggtgttcg tgctgattga tgaggtggag agtctcacag
841ccgcccgaaa tgcctgcagg gcgggcaccg agccatcaga tgccatccgc gtggtcaatg
901ctgtcttgac ccaaattgat cagattaaaa ggcattccaa tgttgtgatt ctgaccactt
961ctaacatcac cgagaagatc gacgtggcct tcgtggacag ggctgacatc aagcagtaca
1021ttgggccacc ctctgcagca gccatcttca aaatctacct ctcttgtttg gaagaactga
1081tgaagtgtca gatcatatac cctcgccagc agctgctgac cctccgagag ctagagatga
1141ttggcttcat tgaaaacaac gtgtcaaaat tgagccttct tttgaatgac atttcaagga
1201agagcgaggg cctcagcggc cgggtcctga gaaaactccc ctttctggct catgcgctgt
1261atgtccaggc ccccaccgtc accatagagg ggttcctcca ggccctgtct ctggcagtgg
1321acaagcagtt tgaagagaga aagaagcttg cagcttacat ctgcccaact ttcttgtaca
1381aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
1441ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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