Construct: ORF ccsbBroad304_02161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017778.1_s304c1, BRDN0000461497
- Derived from:
- ccsbBroadEn_02161
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP7B1 (9420)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02161
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9420 | CYP7B1 | cytochrome P450 family 7 su... | NM_004820.5 | 100% | 100% | |
| 2 | human | 9420 | CYP7B1 | cytochrome P450 family 7 su... | XM_017014002.1 | 91% | 86.9% | (many diffs) |
| 3 | human | 9420 | CYP7B1 | cytochrome P450 family 7 su... | NM_001324112.2 | 87% | 80.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1587
- ORF length:
- 1518
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaggagaa gtgtccgcgg ccacgggccg cttttcgctg gagcggttgg 121 gcctcccggg cctggccctc gccgcggccc tgctgctcct ggccctctgc ttgcttgtcc 181 ggcgcaccag gagacccggt gagcctccat tgataaaagg ttggcttcct tatcttggag 241 tggtcctgaa cttacgaaaa gaccccttaa ggttcatgaa aacacttcaa aagcaacatg 301 gtgacacttt cacagttctt cttggtggaa agtacataac atttatcctg gaccccttcc 361 agtaccagct agtgataaaa aatcataaac aattaagctt tcgagtattt tctaataaat 421 tattagagaa agcatttagc atcagtcagt tgcaaaaaaa tcatgacatg aatgatgagc 481 ttcacctctg ctatcaattt ttgcaaggca aatctttgga catactcttg gaaagcatga 541 tgcagaatct aaaacaagtt tttgaacccc agctgttaaa aaccacaagt tgggacacgg 601 cagaactgta tccattctgc agctcaataa tatttgagat cacatttaca actatatatg 661 gaaaagttat tgtttgtgac aacaacaaat ttattagtga gctaagagat gattttttaa 721 aatttgatga caagtttgca tatttagtat ccaacatacc cattgagctt ctaggaaatg 781 tcaagtctat tagagagaaa attataaaat gcttctcatc agaaaagtta gccaagatgc 841 aaggatggtc agaagttttt caaagcaggc aagatgtcct ggagaaatat tatgtgcacg 901 aggaccttga aataggagca catcatttag gctttctctg ggcctctgtg gcaaacacta 961 ttccaactat gttctgggca atgtattatc ttctgcggca cccagaagct atggcagcag 1021 tgcgtgacga aattgaccgt ttgctgcagt caacaggtca aaagaaaggg tctggatttc 1081 ccatccacct caccagagaa caattggaca gcctaatctg cctagaaagc agcatttttg 1141 aagctttacg actgtcctca tattcaacca ccattcgttt tgttgaggag gatttgactc 1201 tcagttcaga gaccggggac tactgtgtgc gaaagggaga cttggtagcc atctttcctc 1261 cagtcctaca tggtgaccct gaaatctttg aagctccaga ggagtttaga tatgatcgtt 1321 ttatagaaga tggtaagaag aaaaccacct ttttcaaaag agggaaaaag ctgaagtgtt 1381 acctaatgcc gtttggaact ggaaccagca aatgtccagg ccgatttttt gcacttatgg 1441 aaataaaaca attgttggtt atacttttaa cttattttga tttagaaata attgatgata 1501 agcccatagg actaaactac agccgcttgt tgtttggtat tcagtatcca gattctgatg 1561 ttttatttag atacaaagtg aaatctttgc caactttctt gtacaaagtg gttggtaagc 1621 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1681 ggcgcgttaa gtcgacaatc aacctctgga tta