Construct: ORF ccsbBroad304_02187
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011688.1_s304c1, BRDN0000398913
- Derived from:
- ccsbBroadEn_02187
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR1C (9533)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02187
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9533 | POLR1C | RNA polymerase I and III su... | NM_203290.4 | 100% | 100% | |
| 2 | human | 9533 | POLR1C | RNA polymerase I and III su... | NM_001318876.1 | 92.3% | 84.5% | (many diffs) |
| 3 | human | 9533 | POLR1C | RNA polymerase I and III su... | NM_001363658.2 | 88.7% | 88.7% | 921_922ins117 |
| 4 | mouse | 20016 | Polr1c | polymerase (RNA) I polypept... | NM_009085.2 | 86.4% | 93.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1104
- ORF length:
- 1038
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcttctcag gcggtggagg aaatgcggag ccgcgtggtt ctgggggagt 121 ttggggttcg caatgtccat actactgact ttcccggtaa ctattccggt tatgatgatg 181 cctgggacca ggaccgcttc gagaagaatt tccgtgtgga tgtagtacac atggatgaaa 241 actcactgga gtttgacatg gtgggaattg acgcagccat tgccaatgct tttcgacgaa 301 ttctgctagc tgaggtgcca actatggctg tggagaaggt cctggtgtac aataatacat 361 ccattgttca ggatgagatt cttgctcacc gtctggggct cattcccatt catgctgatc 421 cccgtctttt tgagtatcgg aaccaaggag atgaagaagg cacagagata gatactctac 481 agtttcgtct ccaggtcaga tgcactcgga acccccatgc tgctaaagat tcctctgacc 541 ccaacgaact gtacgtgaac cacaaagtgt ataccaggca tatgacatgg atccccctgg 601 ggaaccaggc tgatctcttt ccagagggca ctatccgacc agtgcatgat gatatcctca 661 tcgctcagct gcggcctggc caagaaattg acctgctcat gcactgtgtc aagggcattg 721 gcaaagatca tgccaagttt tcaccagtgg caacagccag ttacaggctc ctgccagaca 781 tcaccctgct tgagcccgtg gaaggggagg cagctgagga gttgagcagg tgcttctcac 841 ctggtgttat tgaggtgcag gaagtccaag gtaaaaaggt ggccagagtt gccaaccccc 901 ggctggatac cttcagcaga gaaatcttcc ggaatgagaa gctaaagaag gttgtgaggc 961 ttgcccgggt tcgagatcat tatatcttct ctgttgagtc aacgggggtg ttgccaccag 1021 atgtgctggt gagtgaagcc atcaaagtac tgatggggaa gtgccggcgc ttcttggatg 1081 aactagatgc ggttcagatg gactgcccaa ctttcttgta caaagtggtt ggtaagccta 1141 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1201 gcgttaagtc gacaatcaac ctctggatta