Construct: ORF ccsbBroad304_02231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009367.1_s304c1, BRDN0000403862
- Derived from:
- ccsbBroadEn_02231
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP6NL (9712)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02231
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9712 | USP6NL | USP6 N-terminal like | NM_014688.4 | 100% | 100% | |
| 2 | human | 9712 | USP6NL | USP6 N-terminal like | NM_001080491.3 | 97.9% | 97.7% | 1_51del;53A>T;55G>A |
| 3 | human | 9712 | USP6NL | USP6 N-terminal like | XM_017016971.1 | 97.8% | 97.6% | 1_50delinsA;54_58delGAAAG |
| 4 | human | 9712 | USP6NL | USP6 N-terminal like | XM_017016973.1 | 97.2% | 97% | 1_67del;69G>T;72_73delAG |
| 5 | human | 9712 | USP6NL | USP6 N-terminal like | XM_006717542.3 | 96.6% | 96.4% | 1_79del;81A>T;84_88delAAAAG |
| 6 | human | 9712 | USP6NL | USP6 N-terminal like | XM_011519762.2 | 96.6% | 96.4% | 1_79del;81A>T;84_88delAAAAG |
| 7 | human | 9712 | USP6NL | USP6 N-terminal like | XM_024448263.1 | 90.4% | 90.4% | 0_1ins237 |
| 8 | human | 9712 | USP6NL | USP6 N-terminal like | XM_011519763.2 | 81.1% | 81.1% | 0_1ins468 |
| 9 | human | 9712 | USP6NL | USP6 N-terminal like | XM_017016972.1 | 81.1% | 81.1% | 0_1ins468 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2550
- ORF length:
- 2484
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttcagaccag gatgtagcac tcaaacttgc ccaggagcga gctgaaatag 121 ttgctaaata tgacagagga cgagaaggtg cagagattga accttgggaa gatgctgatt 181 accttgttta caaagtcaca gatagatttg gctttttaca tgaggaggag ctcccagatc 241 ataatgtggc tgtggaacgg caaaagcacc tggaaattga aagaactacc aaatggctga 301 aaatgctgaa aggatgggaa aaatacaaga acactgaaaa gtttcatagg cgaatttaca 361 aaggaatacc actccagctc agaggtgaag tctgggccct ccttcttgag atccctaaaa 421 tgaaagaaga aacaagggac ctgtatagta aattaaaaca cagagcacgg ggctgttcac 481 ctgacatcag acaaatagac ctggatgtca accgcacatt tcgggaccac attatgttta 541 gagacagata tggtgttaag caacaatcct tattccatgt gcttgctgcc tattctattt 601 ataacacgga agtcgggtat tgtcagggga tgagccagat cacagcttta ctcctcatgt 661 atatgaacga ggaagatgcc ttctgggccc tggtcaaact cttctcaggc cctaaacatg 721 ccatgcatgg cttttttgtc caaggttttc ctaaactctt gaggtttcaa gaacatcatg 781 aaaaaatact gaacaaattt ctgtccaagc ttaagcaaca cttggattct caagaaatct 841 acacaagttt ttacacaatg aaatggtttt ttcagtgttt ccttgatcgt actcccttta 901 cactaaacct cagaatatgg gatatctaca tctttgaagg agaacgagtt cttactgcta 961 tgtcttacac catcttaaaa ttacacaaaa aacatctaat gaaattgtcc atggaagaac 1021 ttgtagaatt ttttcaggag accctggcaa aggatttttt ctttgaagat gattttgtga 1081 tagagcaact tcagatttct atgacagaac taaagcgggc aaagttagac cttccagaac 1141 ctggtaaaga ggatgaatat ccaaagaagc ccttggggca gcttccacct gaacttcagt 1201 cttggggcgt ccatcacttg agcaacggac agaggagcgt gggccggccg agcccgctgg 1261 ccagcggcag gagggagagc ggggcgcccc acaggaggca cgagcactcc ccgcaccccc 1321 agagcaggac cgggacgccc gagagagcac agccgccaag acggaaatcg gtggaggagg 1381 agagcaaaaa gcttaaagat gaggcagatt ttcaaagaaa actcccatcg ggtccacagg 1441 acagttccag gcaatataat cacgcagctg ccaaccaaaa tagcaacgcc acttcaaata 1501 tcaggaagga gtttgtgccc aaatggaata aaccgtcaga cgtctcagct acagagagaa 1561 ctgccaaata caccatggaa ggcaaaggtc gagcagcgca ccccgcgctc gcagttaccg 1621 tcccaggtcc tgccgaggtg cgggtgtcaa acgtgcggcc aaagatgaag gccctggatg 1681 ctgaggacgg gaagcggggc tccactgcat cgcagtacga caacgtgcca ggcccggagc 1741 tggacagcgg cgcttccgtg gaggaggcgc tggaaagggc ttactcccag agcccccggc 1801 atgcccttta ccctcctagc ccgagaaagc acgctgagcc aagttctagt ccatcaaaag 1861 tatccaacaa gtttactttt aaagtacagc ctccaagtca tgcacgatat ccgtcccagc 1921 tagatgggga agcccgaggg ctagctcatc ccccctccta cagcaatccc cccgtttacc 1981 acggaaactc tcccaaacac ttccctactg ccaacagcag ctttgcttct ccacagttta 2041 gccctgggac tcaactgaat ccttccagga gacctcatgg ttctactctt tccgtcagtg 2101 cttctccgga gaaatcttac agccgcccaa gcccccttgt actgccgtct agtcgaatag 2161 aagtcctccc tgttgacact ggtgctgggg gatattcggg caattcaggg tcaccaaaga 2221 atggaaaatt gatcattcca ccagtggatt acttgccaga taacagaaca tggtcagaag 2281 ttagttatac atacagacct gagacgcagg gacaatcatg gacccgagat gctagccgtg 2341 gcaatttacc aaaatactca gcctttcaac tcgcaccctt tcaggaccat ggcctccctg 2401 cagtttctgt agatagtccc gtgagatata aagcttcacc ggccgcagaa gatgccagtc 2461 catctggata tccatattca gggcccccgc ctccagccta ccactacagg aatcgggacg 2521 ggctttccat ccaagagtca gtgttgctgt gcccaacttt cttgtacaaa gtggttggta 2581 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2641 ggtggcgcgt taagtcgaca atcaacctct ggatta