Construct: ORF ccsbBroad304_02238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007836.1_s304c1, BRDN0000405920
- Derived from:
- ccsbBroadEn_02238
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCLAF (9768)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02238
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9768 | PCLAF | PCNA clamp associated factor | NM_014736.6 | 100% | 100% | |
| 2 | human | 9768 | PCLAF | PCNA clamp associated factor | NM_001029989.3 | 47.4% | 40.5% | 127_128ins163;171_195del |
| 3 | human | 9768 | PCLAF | PCNA clamp associated factor | NR_109934.1 | 19.7% | (many diffs) | |
| 4 | mouse | 68026 | Pclaf | PCNA clamp associated factor | NM_026515.2 | 85.5% | 84.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 399
- ORF length:
- 333
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gcggactaaa gcagacagtg ttccaggcac ttacagaaaa gtggtggctg 121 ctcgagcccc cagaaaggtg cttggttctt ccacctctgc cactaattcg acatcagttt 181 catcgaggaa agctgaaaat aaatatgcag gagggaaccc cgtttgcgtg cgcccaactc 241 ccaagtggca aaaaggaatt ggagaattct ttaggttgtc ccctaaagat tctgaaaaag 301 agaatcagat tcctgaagag gcaggaagca gtggcttagg aaaagcaaag agaaaagcat 361 gtcctttgca acctgatcac acaaatgatg aaaaagaata cccaactttc ttgtacaaag 421 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 481 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta