Construct: ORF ccsbBroad304_02273

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005141.1_s304c1, BRDN0000400721
Derived from:
ccsbBroadEn_02273
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AMMECR1 (9949)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02273
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9949 AMMECR1 AMMECR nuclear protein 1 NM_001025580.2 100% 100%
2 human 9949 AMMECR1 AMMECR nuclear protein 1 NM_015365.3 88.8% 88.8% 474_584del
3 human 9949 AMMECR1 AMMECR nuclear protein 1 NM_001171689.2 51.9% 51.9% 0_1ins369;105_215del
4 mouse 56068 Ammecr1 Alport syndrome, mental ret... NM_019496.3 78.4% 81.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
954
ORF length:
888
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggcgggttgc tgcggggtga agaagcagaa actgtccagt tcgcccccct
121ctggctcggg tggcggtggt ggcgcctcct cctcctccca ctgcagcgga gagagccagt
181gccgagctgg ggagctggga ctaggaggcg ccggtacgcg gctcaacggg ctgggaggtc
241taaccggagg aggtagcggc agcggctgta ccctctctcc cccccagggc tgcggcggcg
301gcggcggggg gatcgccctg tcgccacctc cgagctgcgg agtggggacc ctactttcta
361ccccggccgc cgccacctct tcctcaccct cctcatcgtc cgccgcctcg tcctcatcgc
421cgggctcccg gaagatggtg gtgtcagcag agatgtgctg cttttgcttc gatgtgctct
481actgtcacct gtatggatac cagcagcccc ggaccccccg attcaccaac gagccctatg
541cccttaaaga tagccgtttt cccccaatga caagggatga gctgccacgg cttttctgct
601cagtgtctct gctcactaac tttgaagatg tctgtgatta tttggactgg gaggtgggtg
661tacatggcat tagaatagaa ttcatcaatg aaaaaggatc aaaacgcacc gccacctacc
721taccggaggt tgcaaaggag caaggatggg accatataca gaccatagac tccttattga
781ggaaaggagg atacaaagct ccgattacta atgaattcag gaaaaccata aaactgacca
841ggtatcgtag tgaaaagatg accctgagct atgctgaata ccttgctcat cgccagcatc
901atcatttcca aaatggcatt gggcatcccc ttccgccata caaccattat tcctgcccaa
961ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1021cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)