Construct: ORF ccsbBroad304_02283

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014057.1_s304c1, BRDN0000400295
Derived from:
ccsbBroadEn_02283
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DGCR2 (9993)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02283
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9993 DGCR2 DiGeorge syndrome critical ... NM_005137.3 100% 100%
2 human 9993 DGCR2 DiGeorge syndrome critical ... NM_001184781.2 99.4% 99.2% 328_329insGTTTCCTAG
3 human 9993 DGCR2 DiGeorge syndrome critical ... NM_001173533.1 92.5% 92.5% 75_76ins123
4 human 9993 DGCR2 DiGeorge syndrome critical ... NM_001173534.2 92% 91.8% 75_76ins123;205_206insGTTTCCTAG
5 human 9993 DGCR2 DiGeorge syndrome critical ... XR_001755406.2 35.9% (many diffs)
6 human 9993 DGCR2 DiGeorge syndrome critical ... XR_001755405.1 35.8% 1_248del;1051_1175del;2024_4605del
7 human 9993 DGCR2 DiGeorge syndrome critical ... NR_033674.2 34.3% (many diffs)
8 mouse 13356 Dgcr2 DiGeorge syndrome critical ... NM_010048.3 87.7% 93.2% (many diffs)
9 mouse 13356 Dgcr2 DiGeorge syndrome critical ... NM_001109750.1 87.2% 92.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1716
ORF length:
1650
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gcccaaggca gacagcggcg ccttcctgct gctcttcctg ctcgtgctca
121ctgtcaccga gccgctgcgg ccagagctgc ggtgcaaccc tgggcagttt gcgtgtcgca
181gcggcaccat ccagtgcatc cccctcccct ggcagtgtga cggctgggcg acttgcgagg
241atgagagcga cgaagccaac tgtccagaag tgaccgggga ggtgcgtcct catcatggga
301aggaggctgt ggatccgcgg caggggcggg ccagaggagg cgacccttcg cacttccacg
361cggtgaacgt ggcgcagccc gttcgcttca gcagtttcct agggaagtgc ccgacagggt
421ggcaccacta cgaaggcacg gccagctgct accgggtcta cctgagcggg gagaactact
481gggatgccgc gcagacctgc cagcgcctga atggctctct cgccaccttc tccactgacc
541aggagctgcg ctttgtcctg gcccaggaat gggaccagcc cgagcggagc tttggttgga
601aggaccagcg caagttgtgg gttggctatc agtatgttat cactggccgg aaccgctcct
661tggaaggtcg ctgggaggtg gcattcaaag gctcttcaga ggtgttcctg cccccagacc
721ccatctttgc ctcggccatg tctgagaacg acaacgtgtt ctgtgcccag cttcagtgct
781tccatttccc caccctgcgg caccacgacc tccacagctg gcacgccgag agctgctacg
841agaagtcttc atttctgtgt aaaagaagtc aaacatgtgt tgacatcaag gacaacgtgg
901tggatgaagg gttctacttc acccctaagg gggacgaccc atgcctgagc tgcacctgcc
961atggagggga gcctgagatg tgtgtggctg ctctctgtga gaggccccag ggctgccaac
1021agtaccgcaa ggaccccaaa gagtgctgca agttcatgtg tctggaccca gatggcaaca
1081gtctgtttga ctccatggcc agcgggatgc gcctggtcgt cagctgcatc tcctccttcc
1141tcatcctgtc actgctgctc ttcatggtcc accggctgcg ccagcggcgc cgggagcgca
1201tcgagtccct gattggagca aacttgcacc acttcaacct cggccgcagg atccctggct
1261ttgattacgg cccagacggg tttggcacgg gcctcacgcc gctgcatctt tctgacgacg
1321gagagggtgg gactttccat ttccacgacc ctccacctcc ctacacggca tacaagtacc
1381cggacatcgg ccagcccgac gaccctccgc cgccctacga ggcctccatc cacccggaca
1441gtgtgttcta tgaccctgca gacgatgatg cttttgagcc tgtggaggtc agcctgccag
1501cccctgggga tggtgggagt gaaggtgcat tactccggcg cctggagcag cctctgccca
1561ctgcgggggc ctctctggca gacctggaag actctgccga cagcagcagc gccctgctcg
1621tgccccctga ccctgcccag agcgggagca ccccagctgc agaggcactg ccagggggtg
1681gccgccacag ccgcagctcc ctcaatactg tggtgtaccc aactttcttg tacaaagtgg
1741ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1801ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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