Construct: ORF ccsbBroad304_02307
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010106.1_s304c1, BRDN0000405681
- Derived from:
- ccsbBroadEn_02307
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CERT1 (10087)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02307
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10087 | CERT1 | ceramide transporter 1 | NM_031361.3 | 100% | 100% | |
| 2 | human | 10087 | CERT1 | ceramide transporter 1 | XM_017008919.2 | 100% | 100% | |
| 3 | human | 10087 | CERT1 | ceramide transporter 1 | NM_005713.3 | 95.8% | 95.8% | 1109_1186del |
| 4 | human | 10087 | CERT1 | ceramide transporter 1 | XM_006714513.3 | 95.8% | 95.8% | 1109_1186del |
| 5 | human | 10087 | CERT1 | ceramide transporter 1 | XM_011543091.3 | 94.4% | 90.6% | (many diffs) |
| 6 | human | 10087 | CERT1 | ceramide transporter 1 | XM_011543090.3 | 90.6% | 87% | (many diffs) |
| 7 | human | 10087 | CERT1 | ceramide transporter 1 | NM_001130105.1 | 79.5% | 79.5% | 1_384del;1493_1570del |
| 8 | mouse | 68018 | Col4a3bp | collagen, type IV, alpha 3 ... | NM_001164222.1 | 90.2% | 96.4% | (many diffs) |
| 9 | mouse | 68018 | Col4a3bp | collagen, type IV, alpha 3 ... | NM_023420.2 | 86.4% | 92.4% | (many diffs) |
| 10 | mouse | 68018 | Col4a3bp | collagen, type IV, alpha 3 ... | XM_011244694.2 | 66.7% | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1860
- ORF length:
- 1794
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggataatcag agctggaact cgtcgggctc ggaggaggat ccagagacgg 121 agtctgggcc gcctgtggag cgctgcgggg tcctcagtaa gtggacaaac tacattcatg 181 ggtggcagga tcgttgggta gttttgaaaa ataatgctct gagttactac aaatctgaag 241 atgaaacaga gtatggctgc agaggatcca tctgtcttag caaggctgtc atcacacctc 301 acgattttga tgaatgtcga tttgatatta gtgtaaatga tagtgtttgg tatcttcgtg 361 ctcaggatcc agatcataga cagcaatgga tagatgccat tgaacagcac aagactgaat 421 ctggatatgg atctgaatcc agcttgcgtc gacatggctc aatggtgtcc ctggtgtctg 481 gagcaagtgg ctactctgca acatccacct cttcattcaa gaaaggccac agtttacgtg 541 agaagttggc tgaaatggaa acatttagag acatcttatg tagacaagtt gacacgctac 601 agaagtactt tgatgcctgt gctgatgctg tctctaagga tgaacttcaa agggataaag 661 tggtagaaga tgatgaagat gactttccta caacgcgttc tgatggtgac ttcttgcata 721 gtaccaacgg caataaagaa aagttatttc cacatgtgac accaaaagga attaatggta 781 tagactttaa aggggaagcg ataactttta aagcaactac tgctggaatc cttgcaacac 841 tttctcattg tattgaacta atggttaaac gtgaggacag ctggcagaag agactggata 901 aggaaactga gaagaaaaga agaacagagg aagcatataa aaatgcaatg acagaactta 961 agaaaaaatc ccactttgga ggaccagatt atgaagaagg ccctaacagt ctgattaatg 1021 aagaagagtt ctttgatgct gttgaagctg ctcttgacag acaagataaa atagaagaac 1081 agtcacagag tgaaaaggtg agattacatt ggcctacatc cttgccctct ggagatgcct 1141 tttcttctgt ggggacacat agatttgtcc aaaaggttga agagatggtg cagaaccaca 1201 tgacttactc attacaggat gtaggcggag atgccaattg gcagttggtt gtagaagaag 1261 gagaaatgaa ggtatacaga agagaagtag aagaaaatgg gattgttctg gatcctttaa 1321 aagctaccca tgcagttaaa ggcgtcacag gacatgaagt ctgcaattat ttctggaatg 1381 ttgacgttcg caatgactgg gaaacaacta tagaaaactt tcatgtggtg gaaacattag 1441 ctgataatgc aatcatcatt tatcaaacac acaagagggt gtggcctgct tctcagcgag 1501 acgtattata tctttctgtc attcgaaaga taccagcctt gactgaaaat gaccctgaaa 1561 cttggatagt ttgtaatttt tctgtggatc atgacagtgc tcctctaaac aaccgatgtg 1621 tccgtgccaa aataaatgtt gctatgattt gtcaaacctt ggtaagccca ccagagggaa 1681 accaggaaat tagcagggac aacattctat gcaagattac atatgtagct aatgtgaacc 1741 ctggaggatg ggcaccagcc tcagtgttaa gggcagtggc aaagcgagag tatcctaaat 1801 ttctaaaacg ttttacttct tacgtccaag aaaaaactgc aggaaagcct attttgttct 1861 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1921 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1981 ggatta