Construct: ORF ccsbBroad304_02307

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010106.1_s304c1, BRDN0000405681
Derived from:
ccsbBroadEn_02307
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CERT1 (10087)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02307
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10087 CERT1 ceramide transporter 1 NM_031361.3 100% 100%
2 human 10087 CERT1 ceramide transporter 1 XM_017008919.2 100% 100%
3 human 10087 CERT1 ceramide transporter 1 NM_005713.3 95.8% 95.8% 1109_1186del
4 human 10087 CERT1 ceramide transporter 1 XM_006714513.3 95.8% 95.8% 1109_1186del
5 human 10087 CERT1 ceramide transporter 1 XM_011543091.3 94.4% 90.6% (many diffs)
6 human 10087 CERT1 ceramide transporter 1 XM_011543090.3 90.6% 87% (many diffs)
7 human 10087 CERT1 ceramide transporter 1 NM_001130105.1 79.5% 79.5% 1_384del;1493_1570del
8 mouse 68018 Col4a3bp collagen, type IV, alpha 3 ... NM_001164222.1 90.2% 96.4% (many diffs)
9 mouse 68018 Col4a3bp collagen, type IV, alpha 3 ... NM_023420.2 86.4% 92.4% (many diffs)
10 mouse 68018 Col4a3bp collagen, type IV, alpha 3 ... XM_011244694.2 66.7% 71.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1860
ORF length:
1794
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc ggataatcag agctggaact cgtcgggctc ggaggaggat ccagagacgg
121agtctgggcc gcctgtggag cgctgcgggg tcctcagtaa gtggacaaac tacattcatg
181ggtggcagga tcgttgggta gttttgaaaa ataatgctct gagttactac aaatctgaag
241atgaaacaga gtatggctgc agaggatcca tctgtcttag caaggctgtc atcacacctc
301acgattttga tgaatgtcga tttgatatta gtgtaaatga tagtgtttgg tatcttcgtg
361ctcaggatcc agatcataga cagcaatgga tagatgccat tgaacagcac aagactgaat
421ctggatatgg atctgaatcc agcttgcgtc gacatggctc aatggtgtcc ctggtgtctg
481gagcaagtgg ctactctgca acatccacct cttcattcaa gaaaggccac agtttacgtg
541agaagttggc tgaaatggaa acatttagag acatcttatg tagacaagtt gacacgctac
601agaagtactt tgatgcctgt gctgatgctg tctctaagga tgaacttcaa agggataaag
661tggtagaaga tgatgaagat gactttccta caacgcgttc tgatggtgac ttcttgcata
721gtaccaacgg caataaagaa aagttatttc cacatgtgac accaaaagga attaatggta
781tagactttaa aggggaagcg ataactttta aagcaactac tgctggaatc cttgcaacac
841tttctcattg tattgaacta atggttaaac gtgaggacag ctggcagaag agactggata
901aggaaactga gaagaaaaga agaacagagg aagcatataa aaatgcaatg acagaactta
961agaaaaaatc ccactttgga ggaccagatt atgaagaagg ccctaacagt ctgattaatg
1021aagaagagtt ctttgatgct gttgaagctg ctcttgacag acaagataaa atagaagaac
1081agtcacagag tgaaaaggtg agattacatt ggcctacatc cttgccctct ggagatgcct
1141tttcttctgt ggggacacat agatttgtcc aaaaggttga agagatggtg cagaaccaca
1201tgacttactc attacaggat gtaggcggag atgccaattg gcagttggtt gtagaagaag
1261gagaaatgaa ggtatacaga agagaagtag aagaaaatgg gattgttctg gatcctttaa
1321aagctaccca tgcagttaaa ggcgtcacag gacatgaagt ctgcaattat ttctggaatg
1381ttgacgttcg caatgactgg gaaacaacta tagaaaactt tcatgtggtg gaaacattag
1441ctgataatgc aatcatcatt tatcaaacac acaagagggt gtggcctgct tctcagcgag
1501acgtattata tctttctgtc attcgaaaga taccagcctt gactgaaaat gaccctgaaa
1561cttggatagt ttgtaatttt tctgtggatc atgacagtgc tcctctaaac aaccgatgtg
1621tccgtgccaa aataaatgtt gctatgattt gtcaaacctt ggtaagccca ccagagggaa
1681accaggaaat tagcagggac aacattctat gcaagattac atatgtagct aatgtgaacc
1741ctggaggatg ggcaccagcc tcagtgttaa gggcagtggc aaagcgagag tatcctaaat
1801ttctaaaacg ttttacttct tacgtccaag aaaaaactgc aggaaagcct attttgttct
1861acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1921attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1981ggatta

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