Construct: ORF ccsbBroad304_02355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017975.1_s304c1, BRDN0000405626
- Derived from:
- ccsbBroadEn_02355
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLOT1 (10211)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02355
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10211 | FLOT1 | flotillin 1 | NM_005803.4 | 100% | 100% | |
| 2 | human | 10211 | FLOT1 | flotillin 1 | XM_005248780.3 | 100% | 100% | |
| 3 | human | 10211 | FLOT1 | flotillin 1 | XM_017010157.1 | 90.7% | 88.7% | 1_56del;98_99ins68 |
| 4 | human | 10211 | FLOT1 | flotillin 1 | NM_001318875.1 | 88.7% | 88.7% | 208_209ins144 |
| 5 | human | 10211 | FLOT1 | flotillin 1 | XM_006714947.3 | 87.5% | 86.8% | 42_43ins68;49_50ins91 |
| 6 | human | 10211 | FLOT1 | flotillin 1 | XM_017010158.1 | 83.3% | 83.2% | 1_56del;98_99ins167 |
| 7 | mouse | 14251 | Flot1 | flotillin 1 | NM_008027.2 | 89.7% | 98.1% | (many diffs) |
| 8 | mouse | 14251 | Flot1 | flotillin 1 | XM_006523656.3 | 89.7% | 98.1% | (many diffs) |
| 9 | mouse | 14251 | Flot1 | flotillin 1 | XM_006523657.1 | 72.5% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1347
- ORF length:
- 1281
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tttcacttgt ggcccaaatg aggccatggt ggtctccggg ttctgccgaa 121 gccccccagt catggtggct ggagggcgtg tctttgtcct gccctgcatc caacagatcc 181 agaggatctc tctcaacaca ctgaccctca atgtcaagag tgaaaaggtt tacactcgcc 241 atggggtccc catctcagtc actggcattg cccaggtaaa aatccagggg cagaacaagg 301 agatgttggc ggccgcctgt cagatgttcc tggggaagac ggaggctgag attgcccaca 361 ttgccctgga gacgttagag ggccaccaga gggccatcat ggcccacatg actgtggagg 421 agatctataa ggacaggcag aaattctcag aacaggtttt caaagtggcc tcctcagacc 481 tggtcaacat gggcatcagt gtggttagct acactctgaa ggacattcac gatgaccagg 541 actatttgca ctctttgggg aaggctcgaa cagctcaagt ccaaaaagat gcacggattg 601 gagaagcaga ggccaagaga gatgctggga tccgggaagc taaagccaag caggaaaagg 661 tgtctgctca gtacctgagt gagatcgaga tggccaaggc acagagagat tacgaactga 721 agaaggccgc ctatgacatc gaggtcaaca cccgccgagc acaggctgac ctggcctatc 781 agcttcaggt ggccaagact aagcagcaga ttgaggagca gcgggtgcag gtgcaggtgg 841 tggagcgggc ccagcaggtg gcagtgcagg agcaggagat cgcccggcgg gagaaggagc 901 tggaggcccg ggtgcggaag ccagcggaag cggagcgcta caagctggag cgcctagccg 961 aggcagagaa gtcccaacta attatgcagg cggaggcaga agccgcgtct gtgcggatgc 1021 gtggggaagc tgaggccttt gccatagggg cccgagcccg agccgaggct gagcagatgg 1081 ccaagaaggc agaagccttc cagctgtacc aagaggctgc tcagctggac atgctgctag 1141 agaagctgcc ccaggtggca gaggagatca gtggtccctt gacttcagcc aataagatca 1201 cactggtgtc cagcggcagt gggaccatgg gggcagccaa agtgactggg gaagtactgg 1261 acattctaac tcgcctgcca gagagtgtgg aaagactcac aggcgtgagc atctcccagg 1321 tgaatcacaa gcctttgaga acagcctgcc caactttctt gtacaaagtg gttggtaagc 1381 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1441 ggcgcgttaa gtcgacaatc aacctctgga tta