Transcript: Human XM_006714947.3

PREDICTED: Homo sapiens flotillin 1 (FLOT1), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FLOT1 (10211)
Length:
1751
CDS:
336..1460

Additional Resources:

NCBI RefSeq record:
XM_006714947.3
NBCI Gene record:
FLOT1 (10211)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006714947.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000330204 CACGATGACCAGGACTATTTG pLKO_005 639 CDS 100% 13.200 18.480 N FLOT1 n/a
2 TRCN0000029313 ACAGAGAGATTACGAACTGAA pLKO.1 812 CDS 100% 4.950 6.930 N FLOT1 n/a
3 TRCN0000313765 GACTGTGGAGGAGATCTATAA pLKO_005 521 CDS 100% 13.200 10.560 N Flot1 n/a
4 TRCN0000353591 ATAGCTGAAGTTGCCTGAATG pLKO_005 1490 3UTR 100% 10.800 8.640 N FLOT1 n/a
5 TRCN0000029312 GCAGAGAAGTCCCAACTAATT pLKO.1 1074 CDS 100% 13.200 9.240 N FLOT1 n/a
6 TRCN0000330203 GCAGAGAAGTCCCAACTAATT pLKO_005 1074 CDS 100% 13.200 9.240 N FLOT1 n/a
7 TRCN0000330269 TGACTGTGGAGGAGATCTATA pLKO_005 520 CDS 100% 13.200 9.240 N FLOT1 n/a
8 TRCN0000382434 CTCTCCTTGCCAAATAGTTTG pLKO_005 1581 3UTR 100% 10.800 7.560 N FLOT1 n/a
9 TRCN0000382424 GGAAGTACTGGACATTCTAAC pLKO_005 1361 CDS 100% 10.800 7.560 N FLOT1 n/a
10 TRCN0000379906 ACATTGCCCTGGAGACGTTAG pLKO_005 469 CDS 100% 6.000 4.200 N FLOT1 n/a
11 TRCN0000029309 CCAGGACTATTTGCACTCTTT pLKO.1 647 CDS 100% 4.950 3.465 N FLOT1 n/a
12 TRCN0000029310 CCAGGTGAATCACAAGCCTTT pLKO.1 1427 CDS 100% 4.050 2.835 N FLOT1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006714947.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02355 pDONR223 100% 87.5% 86.8% None 42_43ins68;49_50ins91 n/a
2 ccsbBroad304_02355 pLX_304 0% 87.5% 86.8% V5 42_43ins68;49_50ins91 n/a
3 TRCN0000474673 TTAAGCTGGACGTTACACCAGCCA pLX_317 35% 87.5% 86.8% V5 42_43ins68;49_50ins91 n/a
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