Construct: ORF ccsbBroad304_02382
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003487.1_s304c1, BRDN0000404449
- Derived from:
- ccsbBroadEn_02382
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DSCR4 (10281)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02382
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10281 | DSCR4 | Down syndrome critical regi... | NR_147130.1 | 31.7% | 1_105del;460_1114del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 420
- ORF length:
- 354
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gttaatcatc ttgacgagag atgatgaacc ccggatattt accccagaca 121 gtgatgccgc ttcaccagca ttgcactcta cttccccgct tcctgatcct gcctcagctt 181 ctcctctcca cagagaagaa aaaattctgc ctaaagtctg caacatcgtt tcctgcctga 241 gtttcagcct gccagcttct cctacggatt ctggacttgc cagccccaca atcataacca 301 gagaggggca gcaattttgg gcaaaatgtc tgatttggaa ataccaactt tacctccatg 361 ggctccacaa gaaatcagat gggagaaggg acaagcagat aagcgcaagc ccatcaacct 421 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta