Construct: ORF ccsbBroad304_02420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017239.1_s304c1, BRDN0000410280
- Derived from:
- ccsbBroadEn_02420
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDC80 (10403)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02420
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10403 | NDC80 | NDC80 kinetochore complex c... | NM_006101.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1992
- ORF length:
- 1926
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcgcagttca gtttccagcg gtggtgctgg ccgcctctcc atgcaggagt 121 taagatccca ggatgtaaat aaacaaggcc tctatacccc tcaaaccaaa gagaaaccaa 181 cctttggaaa gttgagtata aacaaaccga catctgaaag aaaagtctcg ctatttggca 241 aaagaactag tggacatgga tcccggaata gtcaacttgg tatattttcc agttctgaga 301 aaatcaagga cccgagacca cttaatgaca aagcattcat tcagcagtgt attcgacaac 361 tctgtgagtt tcttacagaa aatggttatg cacataatgt gtccatgaaa tctctacaag 421 ctccctctgt taaagacttc ctgaagatct tcacatttct ttatggcttc ctgtgcccct 481 catacgaact tcctgacaca aagtttgaag aagaggttcc aagaatcttt aaagaccttg 541 ggtatccttt tgcactatcc aaaagctcca tgtacacagt gggggctcct catacatggc 601 ctcacattgt ggcagcctta gtttggctaa tagactgcat caagatacat actgccatga 661 aagaaagctc acctttattt gatgatgggc agccttgggg agaagaaact gaagatggaa 721 ttatgcataa taagttgttt ttggactaca ccataaaatg ctatgagagt tttatgagtg 781 gtgccgacag ctttgatgag atgaatgcag agctgcagtc aaaactgaag gatttattta 841 atgtggatgc ttttaagctg gaatcattag aagcaaaaaa cagagcattg aatgaacaga 901 ttgcaagatt ggaacaagaa agagaaaaag aaccgaatcg tctagagtcg ttgagaaaac 961 tgaaggcttc cttacaagga gatgttcaaa agtatcaggc atacatgagc aatttggagt 1021 ctcattcagc cattcttgac cagaaattaa atggtctcaa tgaggaaatt gctagagtag 1081 aactagaatg tgaaacaata aaacaggaga acactcgact acagaatatc attgacaacc 1141 agaagtactc agttgcagac attgagcgaa taaatcatga aagaaatgaa ttgcagcaga 1201 ctattaataa attaaccaag gacctggaag ctgaacaaca gaagttgtgg aatgaggagt 1261 taaaatatgc cagaggcaaa gaagcgattg aaacacaatt agcagagtat cacaaattgg 1321 ctagaaaatt aaaacttatt cctaaaggtg ctgagaattc caaaggttat gactttgaaa 1381 ttaagtttaa tcccgaggct ggtgccaact gccttgtcaa atacagggct caagtttatg 1441 tacctcttaa ggaactcctg aatgaaactg aagaagaaat taataaagcc ctaaataaaa 1501 aaatgggttt ggaggatact ttagaacaat tgaatgcaat gataacagaa agcaagagaa 1561 gtgtgagaac tctgaaagaa gaagttcaaa agctggatga tctttaccaa caaaaaatta 1621 aggaagcaga ggaagaggat gaaaaatgtg ccagtgagct tgagtccttg gagaaacaca 1681 agcacctgct agaaagtact gttaaccagg ggctcagtga agctatgaat gaattagatg 1741 ctgttcagcg ggaataccaa ctagttgtgc aaaccacgac tgaagaaaga cgaaaagtgg 1801 gaaataactt gcaacgtctg ttagagatgg ttgctacaca tgttgggtct gtagagaaac 1861 atcttgagga gcagattgct aaagttgata gagaatatga agaatgcatg tcagaagatc 1921 tctcggaaaa tattaaagag attagagata agtatgagaa gaaagctact ctaattaagt 1981 cttctgaaga atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2041 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2101 caatcaacct ctggatta