Construct: ORF ccsbBroad304_02453

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017605.1_s304c1, BRDN0000403726
Derived from:
ccsbBroadEn_02453
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SEC23B (10483)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02453
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10483 SEC23B SEC23 homolog B, COPII coat... NM_001172745.3 100% 100%
2 human 10483 SEC23B SEC23 homolog B, COPII coat... NM_006363.6 100% 100%
3 human 10483 SEC23B SEC23 homolog B, COPII coat... NM_032985.6 100% 100%
4 human 10483 SEC23B SEC23 homolog B, COPII coat... NM_032986.4 100% 100%
5 human 10483 SEC23B SEC23 homolog B, COPII coat... XM_017027593.1 100% 100%
6 human 10483 SEC23B SEC23 homolog B, COPII coat... NM_001172746.3 97.6% 97.6% 364_365ins54
7 mouse 27054 Sec23b SEC23 homolog B, COPII coat... NM_001252543.1 90.6% 97.1% (many diffs)
8 mouse 27054 Sec23b SEC23 homolog B, COPII coat... NM_001252544.1 90.6% 97.1% (many diffs)
9 mouse 27054 Sec23b SEC23 homolog B, COPII coat... NM_001252545.1 90.6% 97.1% (many diffs)
10 mouse 27054 Sec23b SEC23 homolog B, COPII coat... NM_019787.4 90.6% 97.1% (many diffs)
11 mouse 27054 Sec23b SEC23 homolog B, COPII coat... XM_017318899.1 90.6% 97.1% (many diffs)
12 mouse 27054 Sec23b SEC23 homolog B, COPII coat... NM_001281816.1 72.7% 77.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2367
ORF length:
2301
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc gacatacctg gagttcatcc agcagaatga agaacgggat ggtgtgcgtt
121ttagttggaa cgtgtggcct tccagccggc tggaggctac aagaatggtt gtacccctgg
181cttgtctcct tactcctttg aaagaacgtc cagacctacc tcctgtacaa tatgaacctg
241tgctttgcag caggccaact tgtaaagctg ttctcaaccc actttgtcag gttgattatc
301gagcaaaact ttgggcctgt aatttctgtt ttcaaagaaa tcagtttcct ccagcttatg
361gaggcatatc tgaggtgaat caacctgccg aattgatgcc ccagttttct acaattgagt
421acgtgataca gcgaggtgct cagtcccctc tgatctttct ctatgtggtt gacacatgcc
481tggaggaaga tgaccttcaa gcactcaaag agtccctgca gatgtccctg agtcttcttc
541ctccagatgc tctggtgggt ctgatcacat ttggaaggat ggtgcaggtt catgagctaa
601gctgtgaagg aatctccaaa agttatgtct tccgagggac caaggattta actgcaaagc
661aaatacagga tatgttgggc ctgaccaagc cagccatgcc catgcagcaa gcacgacctg
721cacaaccaca ggagcaccct tttgcttcaa gcagatttct gcagcctgtt cacaagattg
781atatgaacct cactgatctt cttggggagc tacagaggga cccatggcca gtaactcagg
841ggaagagacc tttgcgatcc actggtgtgg ctttgtccat tgctgttggc ttgctggagg
901gcacttttcc aaacacagga gccaggatca tgctgtttac tggaggtccc cctacccaag
961ggcctggcat ggtggttgga gatgaattaa agattcctat tcgttcttgg catgatattg
1021agaaagataa tgcacgattc atgaaaaagg caaccaagca ctatgagatg cttgctaatc
1081gaacagctgc aaatggtcac tgcattgata tttatgcttg tgcccttgat caaactggac
1141ttttggagat gaagtgttgt gcaaatctta ctggaggcta catggtaatg ggagattctt
1201tcaacacttc tctcttcaag cagacattcc aaagaatctt tactaaagat tttaatggag
1261atttccgaat ggcatttggt gctactttgg acgtaaagac ctctcgggaa ctgaagattg
1321caggagccat tggtccatgc gtatctctga atgtgaaagg accgtgtgtg tcagaaaatg
1381agcttggtgt tggtggcacg agtcagtgga aaatctgtgg cctagatcct acatctacac
1441ttggcatcta ttttgaagtt gtcaatcagc acaacacccc gatcccccaa ggaggcagag
1501gagccatcca gtttgtcacg cattatcagc actccagcac ccagagacgc atccgcgtga
1561ccaccatcgc ccgaaattgg gcagatgtac agagtcagct caggcacata gaagcagcat
1621ttgaccagga ggctgcggca gtgttgatgg cacggcttgg ggtgttccga gcggagtcag
1681aggaggggcc cgatgtgctc cggtggctgg accgacaact catccgactg tgtcaaaagt
1741ttggacagta taacaaagaa gaccccactt cttttaggtt atcagattcc ttttctctat
1801atcctcagtt tatgttccat ctgagaagat ctccatttct tcaagtgttt aacaacagtc
1861ctgatgagtc gtcatattac agacatcatt ttgcccggca ggacctgacc cagtccctca
1921tcatgatcca gcccattctc tactcttact cctttcatgg gccaccagag ccagtactct
1981tggatagcag cagcattcta gctgacagaa ttttgctgat ggatactttc tttcaaattg
2041tcatttatct tggtgagacc atagcccagt ggcgtaaagc tggctaccag gacatgcccg
2101agtatgaaaa cttcaagcac cttctgcagg caccactgga tgatgctcaa gaaattctgc
2161aagcacgctt cccgatgcca cgttacatca acacggagca tggaggcagt caggctcgat
2221tccttttgtc caaagtgaac ccatctcaga cacacaataa cctgtatgct tggggacagg
2281aaactggagc acccatccta actgatgatg ttagcctgca ggtgttcatg gaccatttga
2341agaagctggc tgtctccagt gcctgttacc caactttctt gtacaaagtg gttggtaagc
2401ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
2461ggcgcgttaa gtcgacaatc aacctctgga tta

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