Construct: ORF ccsbBroad304_02636
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013537.1_s304c1, BRDN0000405820
- Derived from:
- ccsbBroadEn_02636
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERG28 (11161)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02636
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11161 | ERG28 | ergosterol biosynthesis 28 ... | NM_007176.4 | 100% | 100% | |
| 2 | mouse | 58520 | Erg28 | ergosterol biosynthesis 28 | NM_021446.2 | 92.1% | 97.8% | (many diffs) |
| 3 | mouse | 58520 | Erg28 | ergosterol biosynthesis 28 | XM_006516117.2 | 92.1% | 97.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 486
- ORF length:
- 420
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ccgtttcctg aatgtgttaa gaagttggct ggttatggtg tccatcatag 121 ccatggggaa cacgctgcag agcttccgag accacacttt tctctatgaa aagctctaca 181 ctggcaagcc aaaccttgtg aatggcctcc aagctcggac ctttgggatc tggacgctgc 241 tctcatcagt gattcgctgc ctctgtgcca ttgacattca caacaagacg ctctatcaca 301 tcacactctg gaccttcctc cttgccctgg ggcatttcct ctctgagttg tttgtctatg 361 gaactgcagc tcccacgatt ggcgtcctgg cacccctgat ggtggcaagt ttctccatcc 421 tgggtatgct ggtcgggctc cggtatctag aagtagaacc agtatccaga cagaagaaga 481 gaaactgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 541 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 601 acctctggat ta