Construct: ORF ccsbBroad304_02677
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013835.1_s304c1, BRDN0000399289
- Derived from:
- ccsbBroadEn_02677
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDAP1 (11333)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02677
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11333 | PDAP1 | PDGFA associated protein 1 | NM_014891.7 | 100% | 100% | |
| 2 | mouse | 231887 | Pdap1 | PDGFA associated protein 1 | NM_001033313.3 | 89.6% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc taaaggagga agaaagggag gccacaaagg ccgggcgagg cagtatacaa 121 gccctgagga gatcgacgcg cagctgcagg ctgagaagca gaaggccagg gaagaagagg 181 agcaaaaaga aggtggagat ggggctgcag gtgaccccaa aaaggagaag aaatctctag 241 actcagatga gagtgaggat gaagaagatg actaccagca aaagcgcaaa ggcgttgaag 301 ggctcatcga catcgagaac cccaaccggg tggcacagac aaccaaaaag gtcacacaac 361 tggatctgga cgggccaaag gagctttcga ggagagaacg agaagagatt gagaagcaga 421 aggcaaaaga gcgttacatg aaaatgcact tggccgggaa gacagagcaa gccaaggctg 481 acctggcccg gctggccatc atccggaaac agcgggagga ggctgcccgg aagaaggaag 541 aggaaaggaa agcaaaagac gatgccacat tgtcaggaaa acgaatgcag tcactctccc 601 tgaataagta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 661 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 721 tcaacctctg gatta