Construct: ORF ccsbBroad304_02723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002511.1_s304c1, BRDN0000461790
- Derived from:
- ccsbBroadEn_02723
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF8 (23133)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02723
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23133 | PHF8 | PHD finger protein 8 | NM_015107.3 | 100% | 100% | |
| 2 | human | 23133 | PHF8 | PHD finger protein 8 | XM_017029362.2 | 100% | 100% | |
| 3 | human | 23133 | PHF8 | PHD finger protein 8 | XM_005261997.4 | 97.5% | 97.6% | 3072_3147delinsG |
| 4 | human | 23133 | PHF8 | PHD finger protein 8 | XM_005261999.1 | 97.5% | 97.6% | 3072_3147delinsG |
| 5 | human | 23133 | PHF8 | PHD finger protein 8 | XM_017029361.2 | 97.5% | 97.6% | 3072_3147delinsG |
| 6 | human | 23133 | PHF8 | PHD finger protein 8 | NM_001184896.1 | 96.6% | 96.6% | 1_108del |
| 7 | human | 23133 | PHF8 | PHD finger protein 8 | XM_005261996.1 | 94.3% | 94.3% | 1_108del;3180_3255delinsG |
| 8 | human | 23133 | PHF8 | PHD finger protein 8 | XM_011530778.1 | 94.3% | 94.3% | 1_108del;3180_3255delinsG |
| 9 | human | 23133 | PHF8 | PHD finger protein 8 | NM_001184897.1 | 87.9% | 87.9% | 1323_1324ins303;2769_2844delinsG |
| 10 | human | 23133 | PHF8 | PHD finger protein 8 | XM_005262000.1 | 85% | 85% | 1_108del;1431_1432ins303;2877_2952delinsG |
| 11 | human | 23133 | PHF8 | PHD finger protein 8 | NM_001184898.2 | 84.5% | 84% | (many diffs) |
| 12 | mouse | 320595 | Phf8 | PHD finger protein 8 | NM_001113354.1 | 90.9% | 94.7% | (many diffs) |
| 13 | mouse | 320595 | Phf8 | PHD finger protein 8 | XM_006528875.3 | 90.9% | 94.7% | (many diffs) |
| 14 | mouse | 320595 | Phf8 | PHD finger protein 8 | XM_006528877.3 | 90.9% | 94.7% | (many diffs) |
| 15 | mouse | 320595 | Phf8 | PHD finger protein 8 | XM_006528880.3 | 90.9% | 94.7% | (many diffs) |
| 16 | mouse | 320595 | Phf8 | PHD finger protein 8 | XM_006528878.3 | 81.4% | 85.6% | (many diffs) |
| 17 | mouse | 320595 | Phf8 | PHD finger protein 8 | XM_006528879.3 | 81.4% | 85.6% | (many diffs) |
| 18 | mouse | 320595 | Phf8 | PHD finger protein 8 | NM_177201.5 | 69.6% | 72.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3138
- ORF length:
- 3072
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcggtgccg gtgtattgcc tctgccggct gccttacgat gtgacccgct 121 tcatgatcga gtgtgacatg tgccaggact ggtttcatgg cagttgtgtt ggtgttgaag 181 aggagaaggc tgctgacatt gacctctacc actgccccaa ctgtgaagtc ttgcatgggc 241 cctccattat gaaaaaacgc cgtggatctt caaaggggca tgatacacac aaggggaaac 301 cagtgaagac cgggagccct acgttcgtca gagagctccg gagtaggact tttgacagct 361 cagatgaagt gattctgaag cccactggaa atcaactgac cgtggaattc ctggaagaaa 421 atagcttcag tgtgcccatc ctggtcctga agaaggatgg gttgggcatg acgctgccct 481 cgccatcatt cactgtgagg gatgttgaac actatgttgg ttctgacaaa gagattgatg 541 tgattgatgt gacccgccag gctgactgca agatgaagct tggtgatttt gtgaaatact 601 attacagcgg gaagagggag aaagtcctca atgtcattag tttggaattc tctgatacca 661 gactttctaa ccttgtggag acaccgaaga ttgttcgaaa gctgtcatgg gtcgaaaact 721 tgtggccaga ggaatgtgtc tttgagagac ccaatgtaca gaagtactgc ctcatgagtg 781 tgcgagatag ctatacagac tttcacattg actttggtgg cacctctgtc tggtaccatg 841 tactcaaggg tgaaaagatc ttctacctga tccgcccaac aaatgccaat ctgactctct 901 ttgagtgctg gagcagttcc tctaatcaga atgagatgtt ctttggggac caggtggaca 961 agtgctacaa gtgttccgtg aagcaaggac agacactttt cattcccaca gggtggatcc 1021 atgctgtgct gacgcctgtg gactgccttg cctttggagg gaacttctta cacagcctta 1081 acatcgagat gcagctcaaa gcctatgaga ttgagaagcg gctgagcaca gcagacctct 1141 tcagattccc caactttgag accatctgtt ggtatgtggg aaagcacatc ctggacatct 1201 ttcgcggttt gcgagagaac aggagacacc ctgcctccta cctggtccat ggtggcaaag 1261 ccttgaactt ggcctttaga gcctggacaa ggaaagaagc tctgccagac catgaggatg 1321 agatcccgga gacagtgcga accgtacagc tcattaaaga tctggccagg gagatccgcc 1381 tggtggaaga catcttccaa cagaacgttg ggaagacgag caatatcttt gggctgcaga 1441 ggatcttccc agccggctcc attcccctaa ccaggccagc ccattccact tcagtgtcca 1501 tgtccaggct gtcactgccc tccaaaaatg gttcaaagaa gaaaggcctg aagcccaagg 1561 aactcttcaa gaaggcagag cgaaagggca aggagagttc agccttgggg cctgctggcc 1621 agttgagcta taatctcatg gacacataca gtcatcaggc actgaagaca ggctctttcc 1681 agaaagcaaa gttcaacatc actggtgcct gcttgaatga ctcagatgac gactcaccag 1741 acttggacct tgatggaaat gagagcccat tggccctatt gatgtctaac ggcagtacga 1801 aaagggtgaa gagtttatcc aaatctcggc gaaccaagat agcaaagaag gtagacaagg 1861 ctaggctgat ggcagaacag gtgatggaag acgaatttga cttggattca gatgatgagc 1921 tgcagattga cgagagattg ggaaaggaga aggcgaccct gataataaga ccaaaatttc 1981 cccggaaatt gccccgtgcg aagccttgct ctgaccccaa ccgagttcgt gaaccaggag 2041 aagttgagtt tgacattgag gaggactata caacagatga ggacatggtg gaaggggttg 2101 aaggcaagct tgggaatggt agtggcgctg gtggcattct tgatctgctc aaggccagca 2161 ggcaggtggg gggacctgac tatgctgccc tcaccgaggc cccagcttct cccagcactc 2221 aggaggccat ccagggcatg ctgtgcatgg ccaacctgca gtcctcatcg tcctcaccgg 2281 ctacctctag cctgcaggcc tggtggactg ggggacagga tcgaagcagt gggagctcca 2341 gcagtgggct gggcacagtg tctaacagtc ctgcttccca gcgcacccca gggaagcggc 2401 ccatcaagcg gccagcatac tggagaaccg agagcgagga ggaggaggag aacgccagtc 2461 tggatgaaca ggacagcttg ggagcgtgct tcaaggatgc agagtatatc tatccttcac 2521 tggagtctga tgatgatgac cctgctttga aatctcgacc caagaaaaag aagaattcag 2581 atgatgctcc atggagtcct aaagcccgcg tgaccccaac tctgccgaag caggaccgtc 2641 ctgtgcgtga ggggacccgg gtagcctcta ttgagacagg tttggctgca gcagctgcaa 2701 agctggccca gcaggagcta cagaaggccc aaaagaagaa atatatcaag aagaagcctt 2761 tgctgaagga ggtagaacag cctcgccctc aagactccaa tctcagtctg acagtaccag 2821 cccccactgt ggctgccaca ccacaacttg tcacctcctc ctcacccctg cctcctcctg 2881 agcctaaaca agaggccctg tcaggaagtc tcgctgacca tgagtacacc gctcgtccca 2941 atgcctttgg catggcccag gcaaaccgca gcaccacacc tatggccccc ggtgtcttct 3001 tgacccagcg gcgcccttca gttggctccc agagcaatca ggcaggacaa ggaaagcgtc 3061 ccaaaaaggg cctggccaca gcaaagcaga gactcggccg tatcctgaaa atccacagaa 3121 atggcaaact acttctgtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 3181 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 3241 agtcgacaat caacctctgg atta