Construct: ORF ccsbBroad304_02733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015229.1_s304c1, BRDN0000461511
- Derived from:
- ccsbBroadEn_02733
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXL7 (23194)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02733
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23194 | FBXL7 | F-box and leucine rich repe... | NM_012304.5 | 100% | 100% | |
| 2 | human | 23194 | FBXL7 | F-box and leucine rich repe... | XM_017009262.2 | 98.1% | 97.7% | (many diffs) |
| 3 | human | 23194 | FBXL7 | F-box and leucine rich repe... | NM_001278317.1 | 90.4% | 90.4% | 0_1ins141 |
| 4 | human | 23194 | FBXL7 | F-box and leucine rich repe... | XM_011513998.1 | 90.4% | 90.4% | 0_1ins141 |
| 5 | mouse | 448987 | Fbxl7 | F-box and leucine-rich repe... | NM_176959.3 | 89.6% | 98.3% | (many diffs) |
| 6 | mouse | 448987 | Fbxl7 | F-box and leucine-rich repe... | XM_006520106.3 | 80.9% | 89.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1542
- ORF length:
- 1473
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggcgcgaac aatggcaaac agtacggcag tgagggcaaa ggcagctcga 121 gcatctcatc tgacgtgagt tcaagtacag atcacacgcc cactaaagcc cagaagaatg 181 tggctaccag cgaagactcc gacctgagca tgcgcacact gagcacgccc agcccagccc 241 tgatatgtcc accgaatctc ccaggatttc agaatggaag gggctcgtcc acctcctcgt 301 cctccatcac cggggagacg gtggccatgg tgcactcccc gcccccgacc cgcctcacac 361 acccgctcat ccggctcgcc tccagacccc agaaggagca ggccagcata gaccggctcc 421 cggaccactc catggtgcag atcttctcct tcctgcccac caaccagctg tgccgctgcg 481 cgcgagtgtg ccgccgctgg tacaacctgg cctgggaccc gcggctctgg aggactatcc 541 gcctgacggg cgagaccatc aacgtggacc gcgccctcaa ggtgctgacc cgcagactct 601 gccaggacac ccccaacgtg tgtctcatgc tggaaaccgt aactgtcagt ggctgcaggc 661 ggctcacaga ccgagggctg tacaccatcg cccagtgctg ccccgaactg aggcgactgg 721 aagtctcagg ctgttacaat atctccaacg aggccgtctt tgatgtggtg tccctctgcc 781 ctaatctgga gcacctggat gtgtcaggat gctccaaagt gacctgcatc agcttgaccc 841 gggaggcctc cattaaactg tcacccttgc atggcaaaca gatttccatc cgctacctgg 901 acatgacgga ctgcttcgtg ctggaggacg aaggcctgca caccatcgcg gcgcactgca 961 cgcagctcac ccacctctac ctgcgccgct gcgtccgcct gaccgacgaa ggcctgcgct 1021 acctggtgat ctactgcgcc tccatcaagg agctgagcgt cagcgactgc cgcttcgtca 1081 gcgacttcgg cctgcgggag atcgccaagc tggagtcccg cctgcggtac ctgagcatcg 1141 cgcactgcgg ccgggtcacc gacgtgggca tccgctacgt ggccaagtac tgcagcaagc 1201 tgcgctacct caacgcgagg ggctgcgagg gcatcacgga ccacggtgtg gagtacctcg 1261 ccaagaactg caccaaactc aaatccctgg atatcggcaa atgccctttg gtatccgaca 1321 cgggcctgga gtgcctggcc ctgaactgct tcaacctcaa gcggctcagc ctcaagtcct 1381 gcgagagcat caccggccag ggcttgcaga tcgtggccgc caactgcttt gacctccaga 1441 cgctgaatgt ccaggactgc gaggtctccg tggaggccct gcgctttgtc aaacgccact 1501 gcaagcgctg cgtcatcgag cacaccaacc cggctttctt cttgccaact ttcttgtaca 1561 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag 1621 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta