Construct: ORF ccsbBroad304_02845

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016164.1_s304c1, BRDN0000461746
Derived from:
ccsbBroadEn_02845
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF6 (24148)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02845
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 24148 PRPF6 pre-mRNA processing factor 6 NM_012469.4 100% 100%
2 human 24148 PRPF6 pre-mRNA processing factor 6 XM_006723769.3 92.2% 92.2% 1303_1304ins219
3 mouse 68879 Prpf6 pre-mRNA splicing factor 6 NM_133701.2 88.7% 98.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2889
ORF length:
2823
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa caagaagaag aaaccgttcc tagggatgcc cgcgcccctc ggctacgtgc
121cggggctggg ccggggcgcc actggcttca ccacgcggtc agacattggg cccgcccgtg
181atgcaaatga ccctgtggat gatcgccatg cacccccagg caagagaacc gttggggacc
241agatgaagaa aaatcaggct gctgacgatg acgacgagga tctaaatgac accaattacg
301atgagtttaa tggctatgct gggagcctct tctcaagtgg accctacgag aaagatgatg
361aggaagcaga tgctatctat gcagccctgg ataaaaggat ggatgaaaga agaaaagaaa
421gacgggagca aagggagaaa gaagaaatag agaaatatcg tatggaacgc cccaaaatcc
481aacagcagtt ctcagacctc aagaggaagt tggcagaagt cacagaagaa gagtggctga
541gcatccccga ggttggcgat gccagaaata aacgtcagcg gaacccacgc tatgagaagc
601tgacccctgt tcctgacagt ttctttgcca aacatttaca gaccggagag aaccatacct
661cagtggatcc ccgacaaact caatttggag gtcttaacac accctatcca ggtggactaa
721acactccata cccaggtgga atgacgccag gactgatgac acctggcaca ggtgagctgg
781acatgaggaa gattggccaa gcgaggaaca ctctgatgga catgaggctg agccaggtgt
841ctgactccgt gagtggacag accgtcgttg accccaaagg ctacctgacg gatttaaatt
901ccatgatccc gacacacgga ggagacatca atgatatcaa gaaggcgcga ctgctcctca
961agtctgttcg ggagacgaac cctcatcacc cgccagcctg gattgcatca gcccgcctgg
1021aagaagtcac tgggaagcta caagtagctc ggaaccttat catgaagggg acggagatgt
1081gccccaagag tgaagatgtc tggctggaag cagccaggtt gcagcctggg gacacagcca
1141aggccgtggt agcccaagct gtccgtcatc tcccacagtc tgtcaggatt tacatcagag
1201ccgcagagct ggaaacggac attcgtgcaa agaagcgggt tcttcggaaa gccctcgagc
1261atgttccaaa ctcggttcgc ttgtggaaag cagccgttga gctggaagaa cctgaagatg
1321ctagaatcat gctgagccga gctgtggagt gctgccccac cagcgtggag ctctggcttg
1381ctctggcaag gctggagacc tatgaaaatg cccgcaaggt cttgaacaag gcgcgggaga
1441acattcctac agaccgacat atctggatca cggctgctaa gctggaggaa gccaatggga
1501acacgcagat ggtggagaag atcatcgacc gagccatcac ctcgctgcgg gccaacggtg
1561tggagatcaa ccgtgagcag tggatccagg atgccgagga atgtgacagg gctgggagtg
1621tggccacctg ccaggccgtc atgcgtgccg tgattgggat tgggattgag gaggaagatc
1681ggaagcatac ctggatggag gatgctgaca gttgtgtagc ccacaatgcc ctggagtgtg
1741cacgagccat ctacgcctac gccctgcagg tgttccccag caagaagagt gtgtggctgc
1801gcgccgcgta cttcgagaag aaccatggca ctcgggagtc cctggaagca ctcctgcaga
1861gggctgtggc ccactgcccc aaagcagagg tgctgtggct catgggcgcc aagtccaagt
1921ggctggcagg ggatgtgcct gcagcaagga gcatcctggc cctggccttc caggccaacc
1981ccaacagtga ggagatctgg ctggcagccg tgaagctgga gtccgagaat gatgagtacg
2041agcgggcccg gaggctgctg gccaaggcgc ggagcagtgc ccccaccgcc cgggtgttca
2101tgaagtctgt gaagctggag tgggtgcaag acaacatcag ggcagcccaa gatctgtgcg
2161aggaggccct gcggcactat gaggacttcc ccaagctgtg gatgatgaag gggcagatcg
2221aggagcagaa ggagatgatg gagaaggcgc gggaagccta taaccagggg ttgaagaagt
2281gtccccactc cacacccctg tggcttttgc tctctcggct ggaggagaag attgggcagc
2341ttactcgagc acgggccatt ttggaaaagt ctcgtctgaa gaacccaaag aaccctgggc
2401tgtggttgga gtccgtgcgg ctggagtacc gtgcggggct gaagaacatc gcaaatacac
2461tcatggccaa ggcgctgcag gagtgcccca actccggtat cctgtggtct gaggccatct
2521tcctcgaggc aaggccccag aggaggacca agagcgtgga tgccctgaag aagtgtgagc
2581atgaccccca tgtgctcctg gccgtggcca agctgttttg gagtcagcgg aagatcacca
2641aggccaggga gtggttccac cgcactgtga agattgactc ggacctgggg gatgcctggg
2701ccttcttcta caagtttgag ctgcagcatg gcactgagga gcagcaggag gaggtgagga
2761agcgctgtga gagtgcagag cctcggcatg gggagctgtg gtgcgccgtg tccaaggaca
2821tcgccaactg gcagaagaag atcggggaca tccttaggct ggtggccggc cgcatcaaga
2881acaccttctg cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
2941tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
3001tcaacctctg gatta

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